四川省77名男男性行为人群HIV-1分子流行病学研究  

Moleculo-epidemiological study of HIV-1/AIDS among 77 men having sex with men in Sichuan province

作　　者：袁丹[1] 冯燎[1] 奚竞婧[1] 罗德维[2] 贾蜀光[3] 赖文红[1] 李恬[1] 余佳[1] 梁姝[1] 魏东兵[1] 周更生[1] 秦光明[1] 于飞 刘红露[1] 

机构地区：[1]四川省疾病预防控制中心艾滋病确证中心实验室,成都610041 [2]达州市疾病预防控制中心病毒检验科,四川达州635002 [3]绵阳市疾病预防控制中心艾滋病确证实验室,四川绵阳621000 [4]成都同乐健康咨询服务中心,四川成都610017

出　　处：《中国艾滋病性病》2011年第4期410-413,447,共5页Chinese Journal of Aids & STD

基　　金：中国第四轮全球基金艾滋病项目(项目编号:CHN-405-G05-H)

摘　　要：目的了解四川省男男性行为人群(MSM)中感染艾滋病病毒Ⅰ型(HIV-1)各亚型的流行情况,探索HIV-1感染规律。方法采集近年来MSM人群中HIV感染者的样本,进行核酸提取、聚合酶链反应(PCR)及基因测序,对所得结果进行pol基因初步鉴定,同时用软件MEGA 4.1进行系统发生分析。结果在HIV感染者较多的成都、绵阳和达州三个地区采集的234份样本中,基因测序成功77份,其中CRF07_BC、CRF01_AE和B亚型分别为42份、24份和11份,各组群内基因离散率分别为(1.56%±1.10%)、(2.56%±0.82%)和(5.63%±2.02%)。结论各亚型组群内的部分感染者来自该亚型同一种毒株感染,CRF07_BC、CRF01_AE和B亚型毒株是当前四川省MSM人群HIV-1的主要流行毒株。Objective To understand HIV-1 subtype prevalence among men who having sex with men（MSM） in Sichuan province and explore rules of HIV-1 infections among them.Methods The samples were collected to extract nucleic acid for PCR and gene sequencing.Their pol genotypes were identified and were analyzed by MEGA4.1.Result In 77 subtype samples collected from Chengdu,Mianyang and Dazhou,the number of CRF07_BC,CRF01_AE and B subtypes were 42,24 and 11 respectively,and the genetic distances were 1.56%±1.10%,2.56%±0.82% and 5.63%±2.02% respectively.Conclusion Some infected persons of different subgroups are infected by the same subtype strains.At present,the main HIV-1 subtypes among MSM in Sichuan province are CRF07_BC,CRF01_AE and B.

关 键 词：艾滋病病毒I型 男男性行为人群 亚型 流行病学 

分 类 号：R512.91[医药卫生—内科学]