A Genetic Linkage Map of Kenaf (Hibiscus cannabinus L.) Based on SRAP, ISSR and RAPD Markers  

作　　者：ZHANG Guang-qing QI Jian-min ZHANG Xiao-chen FNAG Ping-ping SU Jian-guang TAO Ai-fen LAN Tao WU Wei-ren LIU Ai-min 

机构地区：[1]Key Laboratory for Genetics, Breeding and Multiple Utilization of Crops, Ministry of Education, Fuzhou 350002, P.R.China [2]Crop Sciences Institute, Fujian Academy of Agricultural Sciences, Fuzhou 350013, P.R.China [3]College of Life Science, Fujian Agriculture and Forestry University, Fuzhou 350002, P.R. China [4]Institute of Bast Fiber Crops, Chinese Academy of Agricultural Sciences, Changsha 410205, P.R.China [5]Department of Chemistry and Center for Metals in Biocatalysis, University of Minnesota, St. Paul, MN55108, USA

出　　处：《Agricultural Sciences in China》2011年第9期1346-1353,共8页中国农业科学（英文版）

基　　金：supported by the National Natural Science Fundation of China (30571188 and 31000734);the National Agri-Industry Technology Research System for Crops of Bast and Leaf Fiber, China (nycytx-19-E05);the Construction of Germplasm Resources Platform for Bast Fiber Crops in Fujian, China (2010N2002)

摘　　要：Kenaf （Hibiscus cannabinus L.） is one of the most economically important crops for non-wood fiber production. The objective of this study was to establish a genetic linkage map of kenaf with higher density of molecular markers. A semi- wild variety Ga42 and a cultivar Alain kenaf were used as parents to construct an F2 population consisting of 155 plants. The genetic linkage map comprising 134 marker loci was constructed, including 65 sequence-related amplified polymorphism （SRAP）, 56 inter-simple sequence repeat （ISSR）, and 13 randomly amplified polymorphic DNA （RAPD） markers. This map spans 2 108.9 cM and contains 20 linkage groups with an average marker density of 15.7 cM between the adjacent markers.Kenaf （Hibiscus cannabinus L.） is one of the most economically important crops for non-wood fiber production. The objective of this study was to establish a genetic linkage map of kenaf with higher density of molecular markers. A semi- wild variety Ga42 and a cultivar Alain kenaf were used as parents to construct an F2 population consisting of 155 plants. The genetic linkage map comprising 134 marker loci was constructed, including 65 sequence-related amplified polymorphism （SRAP）, 56 inter-simple sequence repeat （ISSR）, and 13 randomly amplified polymorphic DNA （RAPD） markers. This map spans 2 108.9 cM and contains 20 linkage groups with an average marker density of 15.7 cM between the adjacent markers.

关 键 词：KENAF genetic map SRAP ISSR RAPD 

分 类 号：S565.4[农业科学—作物学] S828.2