解毒化瘀药对白血病K562/A02耐药细胞mdr1耐药基因、核因子κB信号影响的研究  

The expressions of multidrug resistance gene1(mdr1) and NF-κB of leukemia K562/A02 cell and the intervention function research of Jeduhuayu Chinese herbal serum

作　　者：马武开[1] 姚血明[1] 唐芳[1] 王莹[1] 黄颖[1] 安阳[1] 黄礼明[1] 

机构地区：[1]贵阳中医学院第二附属医院血液风湿科,贵阳550003

出　　处：《重庆医科大学学报》2011年第8期897-900,共4页Journal of Chongqing Medical University

基　　金：国家自然科学基金资助项目(编号:30860353);贵州省高层次人才科研特助项目

摘　　要：目的:探讨解毒化瘀药含药血清对白血病K562/A02细胞mdr1耐药基因、核因子κB(Nuclear factor kappa B,NF-κB)信号基因表达的影响。方法:采用半定量逆转录聚合酶链反应法检测解毒化瘀药含药血清处理后K562/A02细胞mdr1基因的表达;Western blot法检测NF-κB/P65、NF-κB/P50、IκBα的表达。结果:解毒化瘀药含药血清能够降低K562/A02耐药细胞NF-κB/P65、NF-κB/P50、IκBα表达,降低mdr1耐药基因的表达。结论:解毒化瘀药对K562/A02细胞的耐药逆转作用可能是通过阻断NF-κB信号通路,影响mdr1耐药基因的表达起作用的。Objective：To discuss the influence of Jieduhuayu Chinese herbal serum on the expression of multidrug resistance gene1（Mdr1） of leukemia K562/A02 cell and the expression of NF-κB（nuclear factor kappa B）.Methods：Semi-quantitative reverse transcriptase polymerase chain reaction method was used to examine the expression of Mdr1 gene after K562/A02 cell receiving the treatment of Jieduhuayu Chinese herbal serum;Western blotting technology was used to examine of the expressions of NF-κB/P65,NF-κB/P50,and IκBα.Results：The expressions of NF-κB/P65,NF-κB/P50,and IκBα and the expression of multidrug resistance gene1 of leukemia K562/A02 cell could be decreased by Jieduhuayu Chinese herbal serum.Conclusion：Jieduhuayu Chinese herbal serum can reverse multidrug resistance in leukemia cell by blocking NF-κB signal pathway,and influence the expression of multidrug resistance gene1（mdr1）.

关 键 词：白血病 多药耐药 K562/A02细胞 核因子κB信号 解毒化瘀药 

分 类 号：R557[医药卫生—血液循环系统疾病]