复合组织及血清肿瘤坏死因子α、白细胞介素10表达与大鼠喉移植急性排斥反应的相关性(英文)  

Correlation of tumor necrosis factor-alpha and interleukin-10 expression to acute rejection in complex tissue and serum of rats after laryngeal transplantation

作　　者：袁牧[1] 陈刚[2] 赵华[2] 荆建军[2] 

机构地区：[1]解放军新疆军区机关门诊部,新疆维吾尔自治区乌鲁木齐市830002 [2]解放军兰州军区乌鲁木齐总医院耳鼻喉科,新疆维吾尔自治区乌鲁木齐市830000

出　　处：《中国组织工程研究与临床康复》2011年第31期5701-5706,共6页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:喉移植后致炎细胞因子(肿瘤坏死因子α、γ-干扰素等)和抗炎细胞因子(白细胞介素10、白细胞介素4等)之间的相互作用会发生哪些变化呢?目的:观察肿瘤坏死因子α、白细胞介素10在大鼠喉移植急性排斥反应期移植喉组织的不同部位和血清中的表达变化,评价其血清水平在预测急性排斥反应中的作用。方法:进行Wistar→SD大鼠喉移植,移植后依照注射环孢霉素A剂量不同随机分为3组:0mg组、5mg组及10mg组,以未进行喉移植的SD大鼠为正常对照组。结果与结论:各组移植后第3,7,11天肿瘤坏死因子α、白细胞介素10血清浓度的变化与移植后各相应时间点黏膜上皮及黏膜下层组织中其表达水平的变化均呈正相关。提示,供体喉的高抗原性主要集中于喉的黏膜上皮层及黏膜下层组织;血清肿瘤坏死因子α和白细胞介素10的浓度可以作为预测喉移植术后急性排斥反应的指标。BACKGROUND：It remains unclear whether proinflammatory factor, such as tumor necrosis factor-α （TNF-α）, γ-interferon and anti-inflammatory cytokine, such as interleukin-10 （IL-10）, interleukin-4 （IL-4） levels can change after laryngeal transplantation. OBJECTIVE：To explore the expression of TNF-α and IL-10 in different expressive tissue layers and its relationship during the acute rejection episodes, and to evaluate the role of TNF-α and IL-10 levels in serum for prediction of early acute rejection after laryngeal transplantation. METHODS：Laryngeal heterotopic transplantations were performed in Wistar and Sprague-Dawley rats. According to different dosages of immunodepressant, all recipients were divided into three groups：Group 0 mg, Group 5 mg, and Group 10 mg. Untreated Sprague-Dawley rats served as normal control group. RESULTS AND CONCLUSION：Changes in serum TNF-α and IL-10 levels at postoperation days 3, 7 and 11 were positively correlated with these expressions in the epithelium mucosa and submucosa at various time points after transplantation.These indicate that the high-antigenicity of graft mainly concentrates on the layers of mucosa and submucosa. TNF-α and IL-10 concentrations can serve as indexes for predicting acute rejection after laryngeal transplantation.

关 键 词：肿瘤坏死因子Α 白细胞介素10 喉移植 排斥反应 大鼠 

分 类 号：R617[医药卫生—外科学]