化学去细胞同种异体神经移植修复大鼠骶1神经缺损  

Chemically acellularized nerve allografts for repair of rat S_1 nerve defects

作　　者：李航旭[1] 邹练 刘德忠 张晓毅 张子良 

机构地区：[1]辽宁医学院研究生院,辽宁省锦州市121000 [2]解放军第二炮兵总医院泌尿外科,北京市100088

出　　处：《中国组织工程研究与临床康复》2011年第31期5735-5738,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:自体神经移植是修复周围神经损伤中最常用的方法。目的:探讨化学去细胞神经修复大鼠骶1神经缺损的效果,以期寻找修复周围神经缺损较为理想的方法和材料。方法:取SD大鼠骶1神经,采用化学去细胞法处理大鼠骶1神经的免疫原性成分,使其成为去细胞神经。将Wistar大鼠右侧骶1神经制成1cm缺损模型,用SD大鼠去细胞同种异体神经移植修复大鼠骶1神经的缺损。结果与结论:与术前相比,术后8周逼尿肌漏尿点降低,膀胱最大容积和膀胱顺应性升高(P<0.05)。神经纤维细丝蛋白染色结果显示,右侧经同种异体神经移植修复的神经吻合口断端被染成绿色,神经纤维排列整齐分布均一,再生神经轴突长入远端神经。左侧正常神经形态均一,神经纤维排列整齐分布均一未见轴突长入远端神经。结果表明,化学去细胞同种异体神经的抗原性明显降低,可修复高等哺乳类动物的周围神经损伤。BACKGROUND：Autologous nerve transplantation is the most common method for repair of peripheral nerve injury.OBJECTIVE：To investigate the efficacy of chemically acellularized nerve allografts for repair of rat S1 nerve defects and to search the ideal methods and materials for repair of peripheral nerve injury. METHODS： SD rat S1 nerve was taken and chemically acellularized to remove the immunogenic component. 1 cm long incision was made on the right S1 in SD rats and bridged with the nerve allograft. RESULTS AND CONCLUSION：Compared with prior to surgery, the urinary leakage site of the detrusor muscle was lowered, the maximum volume and compliance of the bladder were significantly increased （P 0.05） at 8 weeks after surgery. Neurofilament staining showed that on the experimental （right） side, neural anastomotic stoma was stained green, nerve fibers were orderly distributed, and regenerated axons grew towards distal nerve;on the left side, nerve morphology was normal, nerve fibers were orderly arranged, and axons did not grow toward distal nerve. These findings suggest that chemically acellularized nerve allografts with obviously decreased antigenicity can repair peripheral nerve injury in high grade mammals.

关 键 词：周围神经 同种异体移植 神经缺损模型 尿动力学 大鼠 

分 类 号：R617[医药卫生—外科学]