自制多脏器保存液对大鼠睾丸一氧化氮合酶的影响  

Effects of self-made multi-organ preservation solution on nitric oxide synthase in cryopreserved rat testicle

作　　者：孙雯[1] 张金萍[1] 揭芳[1] 徐超超[1] 金荣盘[1] 范奇琪[1] 钱申伦[1] 叶波[1] 

机构地区：[1]绍兴文理学院医学院临床医学系,浙江省绍兴市312000

出　　处：《中国组织工程研究与临床康复》2011年第31期5813-5816,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：2010年浙江省大学生科技创新活动计划(新苗人才计划)项目(2010R426002);2009年绍兴市大学生科技创新项目(200912);绍兴文理学院第一批校级优秀教学团队建设成果(20097)~~

摘　　要：背景:在睾丸移植过程中,低温保存和缺血可导致睾丸产生氧自由基而损伤睾丸组织。目的:观察自制多脏器保存液对低温保存大鼠睾丸一氧化氮合酶的影响。方法:采用自制多脏器保存液和UW液低温保存大鼠睾丸,分别于保存24,48,72h时切取睾丸,测定睾丸组织内的总抗氧化能力和一氧化氮合酶活性。结果与结论:自制多器官保存液低温保存各时点大鼠睾丸一氧化氮合酶活性和总抗氧化能力与UW液组比较差异均无显著性意义(P>0.05)。表明自制多脏器保存液能明显减轻低温保存大鼠睾丸氧自由基损伤,其作用与经典的美国威斯康星大学UW保存液基本相当。BACKGROUND：During testis transplantation, cryopreservation and ischemia can lead to production of oxygen free radicals by testis, which damages testis tissue. OBJECTIVE：To investigate the effects of self-made multi-organ preservation solution on nitric oxide synthase （NOS） in cryopreserved rat testicle. METHODS：Rat testicle was cryopreserved by self-made multi-organ preservation solution and UW solution. At 24, 48, and 72 hours after cryopreservation, testicular tissue was taken to determine total antioxidant capacity and NOS activity. RESULTS AND CONCLUSION：There was no significant difference in total antioxidant capacity and NOS activity in rat testicular tissue cryopreserved in the self-made multi-organ preservation solution versus UW solution （P 0.05）. These findings suggest that self-made multi-organ preservation solution can obviously reduce oxygen free radical damage in cryopreserved rat testicular tissue and its effects are equivalent to US solution.

关 键 词：多脏器保存液 低温保存 UW保存液 睾丸 移植 一氧化氮合酶 

分 类 号：R318[医药卫生—生物医学工程]