血管紧张素Ⅱ和血管紧张素-(1-7)对大鼠血管平滑肌细胞肾素(原)受体表达的影响  

Effects of angiotensinⅡ and angiotensin-(1-7) on expression of(pro) renin receptor in rat vascular smooth muscle cells

作　　者：张娜娜[1] 李茂莲[1] 边云飞[1] 高奋[1] 杨志明[1] 杨慧宇[1] 肖传实[1] 

机构地区：[1]山西医科大学第二医院心内科,山西太原030001

出　　处：《中国病理生理杂志》2011年第8期1485-1489,共5页Chinese Journal of Pathophysiology

基　　金：山西省回国留学人员科研资助项目(No.2011-110)

摘　　要：目的:研究血管紧张素Ⅱ(Ang II)和血管紧张素-(1-7)[Ang-(1-7)]对大鼠血管平滑肌细胞(VSMCs)肾素(原)受体[(P)RR]表达的影响。方法:将VSMCs按以下分组:(1)对照组:不加干预因素;(2)不同浓度AngⅡ组:分别加入AngⅡ10、100、1 000 nmol/L;(3)不同浓度Ang-(1-7)组:分别加入Ang-(1-7)10、100、1 000 nmol/L;(4)AngⅡ+losartan(AT1受体拮抗剂)组:losartan 10-6 mol/L预处理30 min后,再加入AngⅡ100 nmol/L;(5)AngⅡ+PD123319(AT2受体拮抗剂)组:先用10-5 mol/LPD123319预处理30 min后,再用终浓度为100 nmol/L AngⅡ;(6)CGP42112A(AT2受体激动剂)组:加入10-7 mol/L CGP42112A。各组用real-time PCR法和Western blotting法检测(P)RR的表达情况。结果:与对照组比,不同浓度AngⅡ可以促进(P)RR mRNA和蛋白的表达,并且呈浓度依赖性(均P<0.01);不同浓度Ang-(1-7)可抑制(P)RR mRNA和蛋白表达(均P<0.01),各浓度之间比较无显著差异(均P>0.05);加入CGP42112A可以促进(P)RR mRNA和蛋白的表达(均P<0.01),与AngⅡ处理组比较,加入PD123319可抑制(P)RR mRNA和蛋白表达(均P<0.01),加入losartan不能抑制(P)RR mRNA和蛋白表达(P>0.05)。结论:AngⅡ可通过AT2受体促进(P)RR mRNA和蛋白表达,而Ang-(1-7)可抑制(P)RR mRNA和蛋白的表达。AIM ： To observe the effects of angiotensin Ⅱ （ Ang H ） and angiotensin - （ 1 - 7 ） [ Ang - （ 1 - 7 ） ] on the expression of （pro） renin receptor [ （P） RR ] in rat vascular smooth muscle cells. METHODS ： The cultured VMSCs were randomly divided into control group, Ang Ⅱ group, Ang - （ 1 - 7 ） group, Ang Ⅱ ＋ losartan （ an AT1 receptor antagonist） group, Ang H ＋ PD123319 （an AT2 receptor antagonist） group and CGP42112A （an AT2 receptor agonist） group. The expression of （P）RR at protein and mRNA levels was detected by Western blotting and real -time PCR, respectively. RESULTS： Compared with control group, Ang Ⅱ distinctly increased and Ang - （ 1 - 7） decreased the expression of （P） RR mRNA and protein in VMSCs in a dose - dependent manner （ P 〈 0. 01 ）. Compared with Ang H group, losartan did not inhibit the expression of （P） RR mRNA and protein in VMSCs induced by Ang IT （ P 〉 0. 05 ）, but PD123319 did （P 〈 0. 01 ）. CGP42112A also induced the expression of （P）RR protein and mRNA in VMSCs （P 〈 0. 01 ）. CONCLUSION： Ang Ⅱ induces the expression of （P） RR in VMSCs by AT2. However, Ang - （ 1 - 7） inhibits the expression of （P）RR in VMSCs.

关 键 词：肾素(原)受体 血管紧张素-(1—7) 血管紧张素Ⅱ 血管平滑肌细胞 

分 类 号：R331.36[医药卫生—人体生理学]