小胶质细胞SFKs在ATP诱导的脊髓背角LTP中的作用(英文)  

Effects of SFKs in microglia on ATP-induced long-term potentiation in spinal dorsal horn

作　　者：宫庆娟[1] 陈金生[1] 黄乔东[1] 卢振和[1] 

机构地区：[1]广州医学院第二附属医院疼痛科,广东广州510260

出　　处：《中国病理生理杂志》2011年第8期1563-1568,共6页Chinese Journal of Pathophysiology

摘　　要：目的:研究Src家族激酶(SFKs)在5'-三磷酸腺苷(ATP)诱导的脊髓背角长时程增强(LTP)中的作用。方法:雄性SD大鼠(250-280 g)。在体电生理记录脊髓腰膨大部背角浅层神经元C-纤维诱发电位,West-ern blotting和免疫组织化学观察脊髓背角SFKs的磷酸化水平和表达部位。结果:ATP应用后30 min和60 min,磷酸化的Src-家族激酶(p-SFKs)的水平明显升高;p-SFKs只表达于小胶质细胞中,星型胶质细胞和神经元中没有表达。脊髓表面应用SFKs抑制剂阻断ATP诱导的LTP。结论:小胶质细胞SFKs可能在ATP诱导的脊髓背角C-纤维诱发电位LTP中发挥重要作用。AIM ： To investigate the effects of Src family kinases （SFKs） on adenosine 5＇- triphosphate （ATP） -induced long- term potentiation （LTP） in the spinal dorsal horn. METHODS： Male Sprague- Dawley rats （250 -280 g） were used in the experiments. Western blotting, electrophysiological recording in spinal dorsal horn in vivo and immunohistochemistry were used in the study. The C -fiber -evoked field potentials were recorded at the superficial layers of spinal dorsal horn at the lumbar enlargement and the phosphorylation level and location of SFKs in spinal dorsal horn were examined by Western blotting and immunohistochemistry. RESULTS： Thirty min and 60 min after ATP application, the levels of phosphorylated SFKs （ p - SFKs） were significantly increased. The p - SFKs were expressed in microglia, but not in astrocytes or neurons. Spinal application of SEK inhibitors prevented ATP - induced LTP. CONCLU- SION： Microglial SFKs may play an important role in ATP - induced LTP of C - fiber evoked field potentials in the spinal dorsal horn.

关 键 词：Src家族激酶类 长时程增强 腺苷三磷酸 脊髓背角 小神经胶质细胞 

分 类 号：R338.2[医药卫生—人体生理学]