猪繁殖与呼吸综合征病毒HN-HW株全基因组分子特征分析  

Genomic characterization of HN-HW strain of porcine reproductive and respiratory syndrome virus

作　　者：马米玲[1] 郝雪峰[1] 关贵全[1] 刘志杰[1] 李有全[1] 刘爱红[1] 

机构地区：[1]中国农业科学院兰州兽医研究所家畜疫病病原生物学国家重点实验室农业部草食动物疫病重点开放实验室甘肃省动物寄生虫病重点实验室,甘肃兰州730046

出　　处：《中国兽医科学》2011年第8期771-777,共7页Chinese Veterinary Science

基　　金：甘肃省自然科学基金项目(0803RJZA043);甘肃省重点项目(1002NKDA035;0801NKDA033)

摘　　要：利用RT-PCR方法扩增得到猪繁殖障碍与呼吸综合征病毒(PRRSV)HN-HW分离株全长基因组的17个片段。拼接后发现该毒株基因组全长为15 334nt(包括poly A尾巴),与国内外14株美洲型PRRSV分离株全序列相似性介于85.3%～99.4%之间,而与欧洲型PRRSV Lelystad Virus(M96262)和SD01-08(DQ489331)分离株全序列相似性仅为59.7%和59.9%。序列分析表明,该毒株包括189nt的5′UTR,14 981nt的蛋白编码区和150nt的3′UTR及14nt的poly(A)尾巴。同美洲标准株ATCC VR-2332相比,Nsp2存在30个氨基酸的缺失。本研究结果补充了PRRSV毒株的基因组信息数据,为深入研究该毒株的遗传与变异及其与生物学特性的关系奠定了基础。To obtain the full-length genome of HN-HW strain,17 fragments of the genome of porcine reproductive and respiratory syndrome virus（PRRSV） were amplified sequenced and assembled. The size of the virus genome was 15 334 nucleotides,including the poly（A） tail. Genomic comparative analysis showed that HN-HW strain shared 85.3% to 99.4% identity with the sequences of 14 North American isolates of domestic and abroad,but only 59.7% and 59.9% identity with that of European prototype of Lelystad Vi- rus（M96262） and SD01-08（DQ489331）. The HN-HW strain genome was consist of a 5r untranslated region（UTR） of 189 nucleotide（nt） ,a protein coding region of 14 981 nt,a 3r UTR of 150 nt,and a 14 bp poly （A） tail. There was a 30 amino acids deletion compared with the North American VR-2332 in the nonstructural protein 2（Nsp2）. Our results enrich information data of PRRSV genome and provide a valuable basis for further study of biological characteristics,heredity and mutation of PRRSV.

关 键 词：猪繁殖与呼吸综合征病毒 HN-HW株 全基因组特征 

分 类 号：S852.651[农业科学—基础兽医学]