不同季节亚成体大熊猫肠道菌群ERIC-PCR指纹图谱分析  

Analysis of the characteristics of intestinal flora with seasonal shift in subadult giant panda by ERIC-PCR fingerprinting

作　　者：王燚[1,2] 何廷美[1,3] 钟志军[1] 王承东[3] 何永果[3] 程安春[1] 李才武[3] 崔婷婷[1] 李德生[3] 彭广能[1] 

机构地区：[1]四川农业大学动物医学院动物疫病与人类健康四川省重点实验室,四川雅安625014 [2]德阳市畜牧食品局,四川德阳618000 [3]中国林业科学研究院大熊猫研究中心四川雅安碧峰峡基地,四川雅安625007

出　　处：《中国兽医科学》2011年第8期778-783,共6页Chinese Veterinary Science

基　　金：大熊猫国际合作资金项目(SD0632);教育部"长江学者和创新团队发展规划"创新团队项目(IRT0848);四川农业大学双支计划项目(00270706)

摘　　要：为探讨不同季节亚成体大熊猫肠道菌群的相似性和稳定性。对采自雅安碧峰峡大熊猫基地3只亚成体大熊猫的粪便细菌总DNA进行了ERIC-PCR指纹图谱分析,并对ERIC-PCR指纹图谱中主带DNA片段进行回收、克隆测序及序列比对分析。结果显示,同一季节不同亚成体大熊猫肠道菌群结构的相似性较高,而不同季节同一亚成体大熊猫肠道菌群结构的相似性较低;亚成体大熊猫肠道菌群的多样性可能受季节影响;大肠杆菌和柠檬酸杆菌为亚成体大熊猫肠道的优势细菌。表明,亚成体大熊猫肠道菌群的多样性在一定程度上与季节有关。To investigate the similarity and stability of subadult giant pandas＇ intestinal flora in four seasons, total DNA of microbial community was extracted from three subadult giant pandas ＇faecal samples and analyzed by ERIC-PCR. Major bands of the ERIC-PCR fingerprint were purified, cloned, sequenced and alignmented. In result,intestinal flora was very similar between different subadult panda individuals in the same season,but tended to be low similarity in different seasons. The season might affect the diversity of subadult giant panda＇s intestinal flora. Escherichia coli and Citrobacter koseri were the predominant bacteria in intestinal flora of subadult panda. Conclusion：The above result showed that the diversity of the subadult giant panda＇s intestinal flora was related with the season in a certain extent.

关 键 词：亚成体大熊猫 肠道细菌 ERIC-PCR 指纹图谱 

分 类 号：S852.612[农业科学—基础兽医学]