山羊关节炎-脑炎病毒陕西分离株全基因组的克隆和序列分析  

Cloning and sequence analysis of the whole genome of caprine arthritis-encephalitis virus Shaanxi strain

作　　者：黄金海[1] 孙跃辉[1] 庄世文[1] 肖华志 张丽霞[1] 

机构地区：[1]天津大学化工学院,天津300072

出　　处：《中国兽医科学》2011年第8期789-793,共5页Chinese Veterinary Science

基　　金：国家自然科学基金项目(30771613)

摘　　要：为了解山羊关节炎-脑炎病毒(CAEV)陕西分离株全基因组的特点及基因演化变化,根据Gen-Bank中登录的CAEV CO株的全基因组序列,设计合成了5对引物,对CAEV陕西株的全基因组进行了PCR扩增,并对扩增产物进行了克隆和测序。结果显示,CAEV陕西株的基因组全长为9 065nt,获得的GenBank登录号为GU120138,包括两端的长末端重复序列,结构蛋白编码基因gag、pol、env和调节蛋白编码基因tat和rev,以及辅助蛋白编码基因vif。核苷酸序列比对表明,CAEV陕西株和CAEV甘肃株的同源性为99.6%,与CAEV CO株的同源性为92.4%,根据全基因组序列和gag基因序列构建的遗传进化分析结果显示,CAEV陕西分离株属于SRLV B2亚型。Five pairs of primers were designed according to the nucleotide sequences of caprine arthritis-encephalitis virus（CAEV） deposited in GenBank,and 5 fragments covering the whole viral genome were obtained and sequenced from infected cells. The whole genome of CAEV Shaanxi strain consists of 9 065 nucleotides,including two LTRs, three structural gene（gag, pol and env gene）, and three regulatory gene （tat, vif and rev gene）. The accession number of CAEV Shaanxi strain in GenBank is GU120138. Comparative analysis with the genomic sequences of CAEV Gansu and CAEV CO strain revealed that CAEV Shaanxi shared 99.6% identity with CAEV Gansu and 92.4% with CAEV CO. Phylogenetic analysis based on complete genome and gag gene, revealed that the CAEV Shaanxi strain had closer relationship with CAEV Gansu strain and CAEV CO strain in comparison with the Italian and Norway strains, and also showed that CAEV Shaanxi strain belonged to SRLV subtype B2.

关 键 词：山羊关节炎-脑炎病毒 基因克隆 基因组解析 进化分析 

分 类 号：S852.659.3[农业科学—基础兽医学]