肺气肿小鼠肺组织CD8＋IL-17＋T细胞变化及作用机制  

The expression and mechanisms of interleukin-17 in CD8＋ T cells of mice with cigarette smokeinduced emphysema

作　　者：段敏超[1] 钟小宁[1] 黄颖[1] 何志义[1] 唐海娟[1] 

机构地区：[1]广西医科大学第一附属医院呼吸内科,南宁530021

出　　处：《中华内科杂志》2011年第9期776-780,共5页Chinese Journal of Internal Medicine

摘　　要：目的观察香烟烟雾暴露肺气肿小鼠肺组织中CD8＋IL-17＋T细胞（Tcl7）的变化，探讨Tcl7在香烟烟雾暴露肺气肿小鼠中的作用及上调的可能机制。方法将40只雄性Balb／c小鼠按随机数字表随机分为A（12周健康对照组）、B（24周健康对照组）、C（12周香烟烟雾暴露肺气肿组）、D（24周香烟烟雾暴露肺气肿组）组，每组10只。香烟烟雾暴露法建立小鼠肺气肿模型，观察小鼠肺气肿的改变，计算平均内衬间隔（Lm）和肺泡破坏指数（DI）；流式细胞术检测小鼠肺组织中CD8＋IL-17＋趋化因子受体6（CCR6）＋细胞及CD8＋IL-17＋CCR6＋细胞；荧光定量PCR法检测小鼠肺组织中维甲酸相关孤独受体（RORTt）、IL-17mRNA表达；ELISA法检测小鼠肺组织匀浆中IL-1B、IL-6、IL-23、转化生长因子（TGF）B及趋化因子CCL20水平，并分析这些指标间的相关性。结果（1）C、D组Lm、DI、Tc17／CD8＋T细胞比例、CD8＋IL-17＋CCR6＋细胞／CD8＋T细胞比例、CD8＋IL-17’CCR6＋细胞／Tc17比例、ROR＇yt和IL-17mRNA相对表达量校正值、趋化因子CCL20浓度、IL-1B、IL-6、IL-23、TGFB水平均明显高于A、B组，以D组增高更为显著，差异均有统计学意义（P值均小于0．05）。（2）C、D组RORγtmRNA与IL-17mRNA相对表达量校正值呈正相关（r=0．651，P〈0．05）。C、D组Tc17／CDs＋T细胞比例与Lm、DI、趋化因子CCL20、IL-1β、IL-6、IL-23、TGFp呈正相关（r值分别为0．734、0．884、0．899、0．531、0．643、0．799、0．637，P〈0．01）。结论香烟烟雾暴露肺气肿小鼠肺内Tc17上调；IL-1β、IL-6、IL-23、TGFβ升高及CCR6、趋化因子CCL20趋化作用促使Tc17上调。Objective To evaluate the expression of Tc17 in a cigarette smoke-induced mice model of emphysema. To explore the probable mechanisms about how Tc17 cells to elevate in lungs of mice. Methods Forty male Balb/c mice were randomly divided into four groups, including control group （12 weeks, C12）, control group （24 weeks, C24）, smoke-exposure group （12 weeks, S12） and smoke- exposure group （24 weeks, $24 ）, 10 mice each group, Emphysema of mice was observed by HE pigmentation. Morphological changes were evaluated by mean linear intercepts （Lm） and destructive index （DI）. The proportion of CDsIL-17 ＋ Tel7, CDs IL-17＋ CC chemokine receptor type 6 （CCR6） ＋ and 6CCR6 ＋ Tc17 ceils in lungs of mice was determined by flow cytometry. The mRNA expressions of retinoid- related orphan nuclear receptor（RORTt） and IL-17 were evaluated by real-time PCR. The levels of IL-1β, IL-6, IL-23, transforming growth factor β （TGFβ） and CC chemokine ligand 20 （CCL20） were tested by ELISA. Correlations among these indexes were analyzed. Results Lm and DI were significantly higher in S12 and S24 than in C12 and C24, S24 in particular （t value 4. 378-15. 188, all P 〈 0. 05） . The percentages of Tc17 in S12 and S24 [ （9.28 ±1.12） %, （ 13.13 ± 3.56） % ] was significantly increased as compared with that in C12 and C24 [ （2. 40 ±0.60）%, （2. 64 ±0. 96）% ], S24 in particular. The mRNA levels of RORγt and IL-17 in S12 and S24 were higher than in C12 and C24, S12 and S24 in particular. There was significant difference （all P 〈 0.05）. The frequency of Tc17 cells had a positive correlation withLm and DI （ r value were 0. 734 and 0. 884 respectively, P 〈 0. 01 ）. The percentages of CDs IL-17 ＋ CCR6 ＋ T cells and CCR6 ＋ Tc17 were significantly elevated in S12 and S24 compared to C12 and C24, S24 in particular （ all P 〈 0. 05 ）. There was positive correlation between Tc17 cell ratio and CCL20 levels （ r = 0. 899, P 〈0. 01 ）. The levels of IL-1β,IL-6

关 键 词：肺气肿 TC17 受体 趋化因子 趋化因子类 白细胞介素类 

分 类 号：R563.3[医药卫生—呼吸系统]