与应力调控相关的基因选择性剪接  

Gene alternative splicing related to stress regulation

作　　者：傅亚[1,2,3] 张徭尧[1,2] 孙姣霞[1,2] 向燕[1,2] 王远亮[1,2] 

机构地区：[1]重庆大学生物流变科学与技术教育部重点实验室,重庆市400044 [2]重庆大学生物工程学院生物材料与仿生工程研究中心,重庆市400044 [3]重庆科技学院生物系,重庆市411331

出　　处：《中国组织工程研究与临床康复》2011年第15期2817-2820,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：国家自然科学基金(30870609;51043004);重庆市自然科学基金(CSTC2009BB4382);重庆市教委科学技术研究项目(Kj091415);九龙坡区科技计划项目(高090109)~~

摘　　要：背景:适当的力学环境是生物体正常生长发育、结构重建以及功能维持的重要因素,也是损伤组织功能性修复的关键因素之一。应力调控基因表达不仅体现在基因的开关或表达水平的调节,还可以体现在转录后的选择性剪接。目的:结合力生长因子介绍选择性剪接这种新的应力调控方式,并推测可能的调控机制。方法:检索PubMed数据库(1964/2010)、CNKI数据库(2000/2009)有关力信号转导和基因选择性剪接方面的综述文章和研究报告,分析二者的联系和可能的调控机制。结果与结论:应力刺激可以导致肌细胞、成骨细胞中的胰岛素样生长因子1基因发生选择性剪接,产生一种新的应力敏感的生长因子力生长因子,这种新型调控方式的机制还不明确,推测与应力导致的剪接小体的位置(位移运动)以及改变了剪接酶(如RNP酶)的位置和空间结构(变形)有关。BACKGROUND：The proper mechanical environment is not only the important factors for organism normal growth and development,structure reestablishment and function maintenance,but also is one of the key factors for functional recovery of injured tissue.The regulation of gene expression by stress does not only reflect the switch or regulation of gene expression,but also is related to alternative splicing after transcription.OBJECTIVE：The introduction of a new stress regulation by alternative splicing combined with growth factor binding,and to predict the presumable regulatory mechanism.METHODS：PubMed database（1964 to 2010） and CNKI database（2000 to 2009） were searched for relative review articles and research reports for aspects of signal transduction and genetic alternative splicing,the linkages between the two and possible regulatory mechanisms were analyzed.RESULTS AND CONCLUSION：Stress stimulation could lead to alternative splicing of insulin like growth factor-1 genes in muscle cells and bone cells,which would produce a new stress-sensitive growth factor mechano-growth factor and reveal a new regulation by stress.The mechanism of regulation is not clear,which may be related to the stress caused by the location of splicing bodies（the displacing movement） and the change of the location and spatial structure（deformation） of splicing enzymes（such as RNP enzyme）.

关 键 词：选择性剪接 生物力学 力学生物学 力生长因子 组织修复 

分 类 号：R318[医药卫生—生物医学工程]