人工合成多肽FGL对神经细胞模型PC12细胞增殖与凋亡的影响(英文)  

Effects of synthetic peptides FG loop on PC12 cells proliferation and apoptosis

作　　者：付洪龙[1] 马学晓[1] 于腾波[1] 陈伯华[1] 李宁[1] 

机构地区：[1]青岛大学医学院附属医院,山东省青岛市266003

出　　处：《中国组织工程研究与临床康复》2011年第15期2847-2850,共4页Journal of Clinical Rehabilitative Tissue Engineering Research

基　　金：Supported by:Research Award Fund for Young Scientists in Shandong Province,No.2007BS03047;Science and Technology Development Plan of Qingdao City,No.06-2-2-6nsh-2~~

摘　　要：背景:FGL是NCAM的核心活性多肽片段,可直接作用于成纤维细胞生长因子受体1,激活NCAM的信号传导途径。目的:观察FGL人工合成多肽联合培养对PC12细胞增殖和凋亡的作用。方法:将培养的PC12细胞分为对照组和实验组,实验组预先加入1%的FGL多肽溶液。分别于培养1,3,5,7,9d采用细胞计数试剂8法检测细胞增殖情况。将PC12细胞分为正常组、实验组和损伤组,损伤组加入H2O2刺激16h。实验组加入H2O2与FGL人工合成多肽刺激16h,流式细胞仪检测细胞凋亡,荧光定量PCR法检测PC12中的核转录因子κBmRNA表达。结果与结论:FGL人工合成多肽与PC12复合培养细胞生长良好,可明显促进PC12细胞的活性并且减低PC12细胞凋亡并可明显降低凋亡模型中PC12细胞核转录因子κB基因的表达。说明FGL多肽可以明显促进PC12细胞增殖,并可以抑制PC12细胞凋亡。BACKGROUND：FG loop（FGL） is a core active peptide fragment of neural cell adhesion molecule（NCAM）,which can directly act on fibroblast growth factor receptor 1（FGFR1） to activate NCAM signal pathway.OBJECTIVE：To observe the effects of synthetic peptides FGL on PC12 cells proliferation and apoptosis.METHODS：①PC12 cells proliferation and apoptosis：The cultured PC12 cells were divided into control group and experiment group.The experimental group was added with 1% FGL peptide solution.The control group was pre-coated with poly-lysine plates.The cells were cultured 1,3,5,7,9 d respectively to detect cell proliferation by using Cell Counting Kit-8.②PC12 apoptosis and nuclear factor kappa B mRNA detection：The PC12 cells were divided into normal group,experimental group and injury group.H2O2 was added into the injury group for 16 hours stimulation.In the experimental group,H2O2 and FGL were used for 16 hours stimulation.The cell apoptosis were detected by flow cytometry;mRNA expression of nuclear factor kappa B was detected by quantitative fluorescent polymerase chain reaction.RESULTS AND CONCLUSION：PC12 cells cocultured with FGL peptide grow well,which indicates that FGL peptides can promote PC12 cell proliferation and inhibit PC12 cell apoptosis,as well as decrease mRNA expression of nuclear factor kappa B.

关 键 词：PC12细胞 FGL多肽 细胞增殖 细胞凋亡 核转录因子ΚB 

分 类 号：R318[医药卫生—生物医学工程]