异位气管移植模型中上皮细胞的增殖与凋亡  

Epithelial proliferation and apoptosis in heterotopic tracheal transplantation model

作　　者：王林毛[1,2] 吴凯 高文[2] 陈昶[2] 鲍方[2] 郑卉[2] 

机构地区：[1]苏州大学医学院,江苏省苏州市215000 [2]同济大学医学院附属上海市肺科医院胸外科,上海市200433 [3]山东省沂源县人民医院普外科,山东省淄博市256100

出　　处：《中国组织工程研究与临床康复》2011年第18期3288-3292,共5页Journal of Clinical Rehabilitative Tissue Engineering Research

摘　　要：背景:异位气管移植中上皮细胞的变化过程尚不清楚,有研究表明在上皮增殖同时伴随有不可逆的细胞死亡,并且气道上皮再生与死亡发生在移植气道闭塞之前。目的:观察异位鼠气管移植过程中气道上皮细胞的增殖与凋亡情况。方法:将异体BALB/c小鼠气管植入BALB/c小鼠颈背部皮下,移植后不注射环孢素A。将BALB/c小鼠气管植入C57BL/6小鼠颈背部皮下,一组不注射环孢素A,另一组全程腹腔注射环孢素A。移植后第3,7,14,21,30天苏木精-伊红染色评价气道上皮的完整性、黏膜下炎症细胞浸润和纤维组织增生情况;测定移植气管中BrdU标记指数及TUNEL阳性细胞数。结果与结论:受体BALB/c小鼠移植后第3天气道上皮细胞轻度损伤丢失,第7～21天,气道上皮逐渐恢复正常,第30天,气道上皮接近正常上皮;BrdU标记指数变化范围为3%～5%;TUNEL阳性细胞数基本无变化。受体C57BL/6小鼠移植后第3天,气道上皮轻度损伤,第7～21天,气管上皮脱落、坏死,开始出现纤维增殖并逐渐加重,第30天时,上皮细胞完全消失,管腔被纤维组织填塞,接近闭塞;移植后第14天,BrdU标记指数达到最高,未注射环孢素A组高于注射环孢素A组(P=0.000),移植后第21,30天,标记指数明显降低;移植后第21天,未注射环孢素A组TUNEL阳性细胞数达到最大值,注射环孢素A组于移植后第14天达最大值。说明异位气管移植上皮增殖同时伴随有不可逆的细胞凋亡,环孢素A可以减轻闭塞性细支气管炎的发病程度,但并不能阻止其发生。BACKGROUND：The process of epithelial cells in heterotopic trachea transplantation remains unclear. The studies demonstrated that epithelial proliferation is accompanied by irreversible cell death, and airway epithelial regeneration and death occurred before airway occlusion transplantation. OBJECTIVE：To observe epithelial proliferation and apoptosis in the process of heterotopic mouse trachea transplantation. METHODS：The allogeneic BALB/c mouse trachea was implanted subcutaneously in the nape of BALB/c mouse. Ciclosporin A （CsA） was not injected after transplantation. The allogeneic BALB/c mouse trachea was implanted subcutaneously in the nape of C57BL/6 mouse. One group was not injected with CsA, the other group was the whole course injected with CsA in abdominal cavity. At 3, 7, 14, 21, 30 days after transplantation, the integrity of airway epithelium, submucosal infiltration of inflammatory cells and fibrous tissue proliferation were evaluated by hematoxylin-eosin staining. BrdU labelling index and TUNEL positive cells in trachea transplantation were determined. RESULTS AND CONCLUSION：At 3 days after receptor BALB/c mouse transplantation, airway epithelial cells suffered from minor injury and loss. At 7-21 days after transplantation, airway epithelial cells recovered normal gradually. Airway epithelium approached to normal epithelium at 30 days after transplantation. The extent of BrdU labelling index was 3%- 5%. The number of TUNEL positive cells was basically unchanged. At 3 days after receptor C57BL/6 mouse transplantation, airway epithelial cells suffered from minor injury. The shedding and necrosis were existed in airway epithelium, at 7-21 days after transplantation;fibrous proliferation was appeared and became more serious gradually. At 30 days after transplantation, epithelial cells completely disappeared, lumens were filled by fibrous tissue, closed to occlusion. The peak of BrdU labelling index was at 14 days after transplantation. The group of not injected with CsA was higher than group

关 键 词：气管移植 同种异体移植 闭塞性气道疾病 同系移植 环孢素A 器官移植 

分 类 号：R617[医药卫生—外科学]