电化学发光法测定HBV标志物与荧光定量PCR-HBV-DNA结果的分析比较  

Analysis and comparison of HBV marker determined by electrochemiluminescence and PCR-HBV-DNA by fluorescent quantification

作　　者：高娅[1] 王婷[2] 

机构地区：[1]河南省嵩县人民医院检验科,河南嵩县471400 [2]郑州大学第二附属医院检验科,河南郑州450014

出　　处：《中国医药科学》2011年第17期158-159,共2页China Medicine And Pharmacy

摘　　要：目的探讨HBV标志物与HBV-DNA的相关性,更加准确地了解患者HBV感染、病毒复制和传染性情况,为临床制定合理的治疗方案、疗效观察和预后评估提供依据。方法用电化学发光法测定HBV标志物,用实时荧光定量PCR方法检测HBV-DNA。结果 HBV标志物模式1～5均有HBV-DNA阳性检出,其中模式4的检出率最高(81.7%),而且DNA含量也最高;模式6～9均为DNA阴性。在乙肝五项指标中,HBeAg的定量结果与HBV-DNA含量相关性最大(r=0.713)。结论 HBV标志物和HBV-DNA既相关又有所不同,应将两者结合起来并相互补充才能更好的反映患者HBV病毒复制和传染性情况。Objective To explore the relativity of HBV marker and HBV-DNA,and further know patient＇s HBV infection,virus replication and infectivity,and then to provide the basis on the treatment method,effectiveness observation and prognosis estimation for clinic.Methods HBV markers were determined by electro-chemiluminescenc and HBV-DNA by real-time fluorescent quantification.Results The pattern 1 ～ 5 of HBV marker had HBV-DNA positive result,among which pattern 4 was the maximum detectable rate;while all of the pattern 6 ～ 9 had HBV-DNA negative result.The quantification of HBeAg,among 5 HBV markers,had the maximum relativity to HBV-DNA content（r=0.713）.Conclusion HBV marker and HBV-DNA are relative,but not the same,so the two should be combined to reflect better the patient＇s HBV replication and infectivity.

关 键 词：HBV标志物 PCR-HBV-DNA 电化学发光法 

分 类 号：R512.62[医药卫生—内科学]