Pien Tze Huang(片仔癀)-Induced Apoptosis in Human Colon Cancer HT-29 Cells Is Associated with Regulation of the Bcl-2 Family and Activation of Caspase 3  

作　　者：林久茂 魏丽慧 陈友琴 刘献祥 洪振丰 Thomas J.Sferra 彭军 

机构地区：[1]Fujian Academy of Integrative Medicine [2]Fujian Key Laboratory of Integrative Medicine on Geriatrics,Fujian University of Traditional Chinese Medicine [3]Department of Pediatrics,The University of Oklahoma Heatth Sciences Center,Oklahoma,City,OK 73190,USA [4]Department of Biochemistry and Molecular Biology,The University of Oklahoma Health Sciences Center,Oklahoma City,OK 73190,USA [5]Fujian Academy of Integrative Medicine,Fuzhou 350108,China [6]Fujian Key Laboratory of Integrative Medicine on Geriatrics,Fujian University of Traditional Chinese Medicine,Fuzhou 350108,China [7]Postdoctor Workstation,Zhangzhou Pien Tze Huang Pharmaceutical Co.,Ltd.

出　　处：《Chinese Journal of Integrative Medicine》2011年第9期685-690,共6页中国结合医学杂志（英文版）

基　　金：Supported by the National Natural Science Foundation of China(No.81073097);the Developmental Fund of CHEN Ke-ji Integrative Medicine(No.CKJ 2011001);the Natural Science Foundation of Fujian Province of China(No.2010J01195)

摘　　要：Objective：To investigate the cellular effects of Pien Tze Huang（片仔癀,PZH） in the HT-29 human colon carcinoma cell line.Methods：The viability of HT-29 cells was determined by MTT assay.A fluorescence-activated cell sorting（FACS） analysis with annexin-V/propidium iodide（PI） and JC-1 staining were performed to determine cell apoptosis and the loss of mitochondrial membrane potential,respectively.Activation of caspase 3 was evaluated by a colorimetric assay.The mRNA expression levels of Bcl-2 and Bax were measured by reverse transcription polymerase chain reaction（RT-PCR）.Results：PZH,in a dose- and time-dependent manner,reduced viability and induced apoptosis of HT-29 cells.Moreover,PZH treatment resulted in the collapse of the mitochondrial membrane potential,activation of caspase 3,and an increase in the Bax/Bcl-2 ratio.Conclusion：PZH inhibits the growth of HT-29 cells by inducing cancer cell apoptosis via regulation of the Bcl-2 family and activation of caspase 3,which may,in part,explain its anticancer activity.Objective：To investigate the cellular effects of Pien Tze Huang（片仔癀,PZH） in the HT-29 human colon carcinoma cell line.Methods：The viability of HT-29 cells was determined by MTT assay.A fluorescence-activated cell sorting（FACS） analysis with annexin-V/propidium iodide（PI） and JC-1 staining were performed to determine cell apoptosis and the loss of mitochondrial membrane potential,respectively.Activation of caspase 3 was evaluated by a colorimetric assay.The mRNA expression levels of Bcl-2 and Bax were measured by reverse transcription polymerase chain reaction（RT-PCR）.Results：PZH,in a dose- and time-dependent manner,reduced viability and induced apoptosis of HT-29 cells.Moreover,PZH treatment resulted in the collapse of the mitochondrial membrane potential,activation of caspase 3,and an increase in the Bax/Bcl-2 ratio.Conclusion：PZH inhibits the growth of HT-29 cells by inducing cancer cell apoptosis via regulation of the Bcl-2 family and activation of caspase 3,which may,in part,explain its anticancer activity.

关 键 词：Pien Tze Huang Chinese medicine colon cancer apoptosis HT-29 cells 

分 类 号：R285[医药卫生—中药学]