雷马Ⅱ型贴膏穴位贴敷对胶原性关节炎大鼠的疗效及作用机制探讨  

Experimental study on effects of acupoint application with Leima type Ⅱ plaster on collagen-induced arthritis in rats

作　　者：李鹏[1] 方剑乔[2] 周亚锋[3] 

机构地区：[1]浙江中医药大学附属第二医院推拿科,杭州310005 [2]浙江中医药大学,杭州310053 [3]浙江省人民医院

出　　处：《中国针灸》2011年第9期821-826,共6页Chinese Acupuncture & Moxibustion

基　　金：浙江省科技厅重点科研项目:2005 C 23012

摘　　要：目的:观察雷马Ⅱ型贴膏穴位贴敷对胶原性关节炎(collagen-induced arthritis,CIA)大鼠的治疗作用,探讨其作用机制。方法:48只Wistar大鼠背部正中线皮内多点注射牛Ⅱ型胶原诱发CIA模型,随机分为模型对照组(A组)、基质对照组(B组)、低浓度贴膏穴位贴敷组(C组)和高浓度贴膏穴位贴敷组(D组),每组12只。C、D组分别贴敷低、高浓度雷马Ⅱ型贴膏,穴取"身柱""至阳""命门",每日1次,每次贴敷约15 h,治疗时间从造模后第1天开始至第30天结束;B组贴敷方法同C、D组,但仅使用不含药物的基质贴膏;A组不施加任何处理。观察病变关节的形态及病理组织变化等。结果:C组大鼠足跖肿胀度在治疗30 d后较同期B组显著降低(P<0.01),血清抗牛Ⅱ胶原抗体水平在治疗15 d后较同期A组显著降低(P<0.05),关节滑膜细胞增殖反应较同期A组和B组显著降低(均P<0.01)。D组大鼠足跖肿胀度在治疗25 d后、关节炎指数在治疗20 d后、大鼠足爪病理损伤和关节滑膜细胞增殖反应较同期A组和B组均显著降低(P<0.01,P<0.05),血清抗牛Ⅱ胶原抗体水平在治疗15 d后较同期A组显著降低(P<0.05)。D组大鼠足跖肿胀度和关节炎指数在治疗25 d后较同期C组大鼠亦显著降低(P<0.05,P<0.01)。结论:雷马Ⅱ型贴膏穴位贴敷对CIA大鼠具有较好的治疗作用,高浓度贴膏疗效优于低浓度贴膏,其作用机制可能是通过降低血清抗牛Ⅱ胶原抗体水平起到抗炎和免疫抑制的作用。Objective To observe the therapeutic effect of acupoint application with Leima type Ⅱ plaster on collagen-induced arthritis（CIA） in rats and probe its mechanism.Methods Bovine typeⅡcollagen was injected intradermally into the middle line of the back to induce CIA model with 48 Wistar rats.Then the rats were randomly divided into a model control group（group A）,a matrix control group（group B）,acupoint application group with plaster of low concentration（group C） and high concentration plaster group（group D）,12 rats in each group.Group C and group D were treated with low and high concentration of Leima type Ⅱ plaster,and ＂Shenzhu＂（GV 12）,＂Zhiyang＂（GV 9） and ＂Mingmen＂（GV 4） were selected,each application for about 15 hours,once each day for 30 days.Group B was used the same method of acupoint application except using non-drug matrix plaster,and group A was not given any treatment.The morphous and the histopathological changes of affection joint were observed.Results The paw edema volume after 30 days treatment in group C was significantly lower than that in group B（P〈0.01）,and the anti-typeⅡcollagen antibody level after 15 days treatment in group C was significantly lower than that in group A（P〈0.05）,and the synoviocytes proliferation of the knee joint in group C was significantly lower than that in group A and group B（both P〈0.01）.The paw edema volume after 25 days treatment,arthritic index after 20 days treatment,pathological change of the paw and the synoviocytes proliferation of the knee joint in group D were significantly lower than those in group A and group B（P〈0.01,P〈0.05）,and the anti-typeⅡcollagen antibody level after 15 days treatment in group D was significantly lower than that in group A（P〈0.05）,and the paw edema volume and the arthritic index after 25 days treatment in group D were significantly lower than those in group C（P〈0.05,P〈0.01）.Conclusion Acupoint application with Leima type Ⅱ plaster has a good ther

关 键 词：胶原性关节炎 雷马Ⅱ型贴膏 穴位贴敷 模型 大鼠 

分 类 号：R244.9[医药卫生—针灸推拿学]