波纹唇鱼血细胞显微结构和血液生化指标  

Microstructure of Peripheral Blood Cell and Serum Biochemical Indices of Cheilinus undulatus

作　　者：廖光勇[1,2] 区又君[1] 李加儿[1] 

机构地区：[1]中国水产科学研究院南海水产研究所,广东广州510300 [2]上海海洋大学水产与生命学院,上海201306

出　　处：《海洋科学进展》2011年第3期379-385,共7页Advances in Marine Science

基　　金：国家高技术研究发展计划项目--名贵海水鱼类苗种规模化繁育技术(2006AA10A414)

摘　　要：以wright’s和Giemsa双重染色法对波纹唇鱼(Cheilinus undulatus)外周血细胞的形态进行显微观察研究并计数,同时测定各类血细胞的大小及血液的生化指标。血涂片观察发现:波纹唇鱼的外周血细胞可区分出红细胞、嗜中性粒细胞、单核细胞、淋巴细胞、血栓细胞等五种血细胞,未观察到嗜碱性粒细胞、嗜酸性粒细胞。在外周血液中还观察到核影、正在分裂的红细胞、幼稚红细胞和降解的红细胞。各类血细胞胞体由大到小的次序依次为单核细胞、嗜中性粒细胞、红细胞、大淋巴细胞、血栓细胞、小淋巴细胞。血浆中红细胞为(3.15±0.79)×10^(12)/L,白细胞为(1.42±0.71)×10^(10)/L,红细胞数远远多于白细胞。血液生化指标与其他鱼类相比有一定差异,表明鱼类血液的生化成分因种和生活环境而异。The microscopic observation and count are conducted for the peripheral blood cells of C. undulatus by means of the Wright＇s and Giemsa double dye method. The serum biochemical indices of C. undulatus , the sizes of the various blood cells are determined. From the stained smears, five blood cell types i. e. erythrocyte, neutrophil, monocyte, lymphocyte and thrombocyte are detected. Neither eosinophilic granulocyten nor basophilic granulocyte is found but, some nuclear shadow of erythrocytes, immature erythrocytes, direct dividing or dissolving erythrocytes. Among these cells, the sizes are put into an order from large to small as follows monocyte, neutrophil, erythrocyte, large lymphocyte, thrombocyte and small lymphlcyte. The erythrocyte and leucocyte concentrations in the blood are （3.15±0.79）×10^12/L and （1.42±0.71）×10^10/L respectively. The number of erythrocyte is much more than the leucocyte in the blood. The serum biochemical indices are different from other fish species. Therefore it is indicated that the serum biochemical indices for various fishes depend upon their species and their inhabiting environment.

关 键 词：波纹唇鱼 血液 外周血细胞 显微结构 生化指标 

分 类 号：Q952[生物学—动物学]