盐酸小檗碱抗白念珠菌体外药敏实验研究  

In vitro susceptibility testing of Candida albicans to berberine hydrochloride

作　　者：竺璐[1] 胡丽华[1] 徐新美[1] 

机构地区：[1]浙江省皮肤病防治研究所皮肤科,浙江德清313200

出　　处：《中华皮肤科杂志》2011年第9期661-662,共2页Chinese Journal of Dermatology

摘　　要：目的探讨盐酸小檗碱（berbefine，BBR）对白念珠菌的体外抗真菌活性。方法采用美国临床实验室标准化委员会（现称美国临床实验室标准化研究所，CLSI）制订的M27-A方案中的酵母菌微量稀释法，测定BBR、氟康唑、BBR联合氟康唑对30株临床分离白念珠菌的抗菌活性。结果盐酸小檗碱对白念珠菌MIC值的几何均数（G）为248．18mg／L。与320、160、80mg／LBBR联合后，氟康唑MIC值的几何均数从2．64mg／L降为0．03、0．35、1．35mg/L。两药对26．7％的白念菌株有协同抗菌作用，其中包括3株耐氟康唑菌，对56．7％的菌株表现为相加作用，对16．7％的菌株为无关作用，未观察到拮抗作用。结论BBR在体外对白念珠菌具有抗真菌作用并能增加其对氟康唑的敏感性。Objective To test the antifungal activity of berberine （BBR） against Candida albicans in vitro. Methods According to the National Committee for Clinical Laboratory Standards （NCCLS） M27-A pro- tocol, broth microdilution test was performed to assess the susceptibility of 30 clinical isolates of Candida albicans to BBR and fluconazole alone or in combination. Results The geometric mean minimum inhibitory concentra- tion （MIC） was 248.18 mg/L and 2.64 mg/L for BBR and flnconazole alone, respectively. When combined with BBR of 320, 160 and 80 rag/L, the geometric mean MIC decreased to 0.03, 0.35 and 1.35 mg/L respectively for fluconazole. Fluconazole and BBR showed synergistic effects in 26.7% of these isolates including 3 flnconazole- resistant strains, additive effects in 56.7%, indifferent effects in 16.7%, antagonistic effects in none. Conclu- sion In vitro, BBR has antifungal activity against Candida albicans and can enhance the activity of flucona- zole against Candida albicans.

关 键 词：盐酸小檗碱 白念珠菌 体外药敏 美国临床实验室标准化委员会 实验研究 体外抗真菌活性 协同抗菌作用 几何均数 

分 类 号：R446.5[医药卫生—诊断学]