一次力竭离心运动后大鼠骨骼肌HSP70的变化及针刺对其影响  

The Changes of HSP70 in Skeletal Muscle after an Exhaustive Eccentric Exercise and the Effects of Acupuncture

作　　者：李俊平[1] 马延超[2] 张炜[3] 

机构地区：[1]北京体育大学,北京100084 [2]洛阳师范学院体育学院,河南洛阳471022 [3]包头师范学院体育学院,内蒙古包头041030

出　　处：《北京体育大学学报》2011年第8期44-47,共4页Journal of Beijing Sport University

基　　金：北京体育大学校管课题"一次力竭离心运动后大鼠骨骼肌热休克蛋白的变化及针刺对其影响"(课题号:2009A017)

摘　　要：目的:观察一次力竭离心运动后大鼠腓肠肌HSP70表达的变化,及针刺对运动后不同恢复时间骨骼肌HSP70表达的影响。方法:雄性SD大鼠,分为安静对照组,单纯运动组和运动加针刺组。除对照组外,其他大鼠均进行一次力竭离心运动。单纯运动组运动后不做处理,运动加针刺组在运动后立即进行针刺,具体方法为从远端斜刺(进针角度为30°)穿过腓肠肌肌腹,并留针5 min。在运动后即刻、恢复12 h、24 h取大鼠腓肠肌进行分析。用免疫荧光组织化学法和western blot检测HSP70蛋白的表达。结果:1)一次性力竭离心运动后即刻、12 h、24 h组腓肠肌HSP70蛋白表达水平均增高,与对照组有显著性差异(P<0.05),HSP70蛋白表达于运动后即刻增加最明显,随后逐渐下降。2)一次性力竭离心运动后对腓肠肌进行针刺,仅12 h组HSP70蛋白表达高于对照组,有显著性差异(P<0.05)。3)一次性力竭离心运动后,相同时间的非针刺组与针刺组比较,针刺组在运动后即刻和24 hHSP70蛋白表达明显低于非针刺组,有显著性差异(P<0.05)。4)一次力竭离心运动后,非针刺组HSP70蛋白在细胞胞质内广泛表达,针刺后即刻HSP70蛋白主要在细胞膜和细胞核表达,随后逐渐分布到细胞质。结论:1)一次力竭离心运动后即刻即可诱导大鼠腓肠肌HSP70蛋白高表达,随恢复时间的延长HSP70蛋白表达下降。2)一次力竭离心运动后针刺骨骼肌,可抑制运动诱导的HSP70蛋白高表达。Purpose：The purpose of this study was to observe the changes of HSP70 expression in rat gastrocnemius muscle after an exhaustive eccentric exercise,and to explore the effects of acupuncture on HSP70 expression in skeletal muscle after exercise.Method：Male SD rats were randomly divided into four groups： control group,non-acupuncture group and acupuncture group after an exhaustive eccentric exercise.The rats of non-acupuncture and acupuncture group were carried out an exhaustive eccentric exercise.After the exercise,the rats of acupuncture group were handled acupuncture on the gastrocnemius vertically from the further side（the angle of the needle was 30 degree） and through the belly of gastrocnemius where the needle stayed for 5 minutes.Then the gastrocnemius was obtained from rats of different groups.The expression of HSP70 of gastrocnemius was detected by western blot and immunofluorescence histochemical method.Results：1）The expression of HSP70 of gastrocnemius in non-acupuncture group were increased markedly immediately,12 hours and 24 hours after exhaustive eccentric exercise（P0.05）.2）The expression of HSP70 of gastrocnemius in acupuncture group were increased markedly only 12 hours after exhaustive eccentric exercise（P0.05）.3）The expression of HSP70 of gastrocnemius in acupuncture group was significantly lower than non-acupuncture group immediately and 24 hours after exhaustive eccentric exercise（P0.05）.4）The expression of HSP70 of gastrocnemius in non-acupuncture group mainly located in cytoplasm.The expression of HSP70 of gastrocnemius in acupuncture group mainly located in nucleus immediately after exhaustive eccentric exercise,and then transferred to cytoplasm gradually.Conclusions：1）Immediately after an exhaustive eccentric exercise,HSP70 expression of rat gastrocnemius were induced markedly,and then decreased gradually.2） HSP70 expression of rat gastrocnemius induced by an exhaustive eccentric exercise might be inhibited by acupuncture.

关 键 词：针刺 离心运动 骨骼肌 热休克蛋白70 

分 类 号：G804.2[文化科学—运动人体科学]