Synthesis and characteristics of a novel artificial hapten using the copper mercaptide of penicillenic acid from penicillin G for immunoassay of heavy metal ions  

作　　者：XU Wu XIE Peng FAN LiuYin CAO ChengXi XI Tao ZHOU Pei 

机构地区：[1]Laboratory of Analytical Biochemistry & Bio-separation, Key Laboratory of Microbiology of Ministry of Education of China, School of Life Science and Biotechnology, Shanghai Jiao Tong University, Shanghai 200240, China [2]School of Agriculture and Biology, Shanghai Jiao Tong University, Shanghai 200240, China

出　　处：《Science China(Life Sciences)》2011年第9期813-821,共9页中国科学（生命科学英文版）

基　　金：supported by the National Natural Science Foundation of China (Grant No. 30821005);National High Technology Research and Development Program of China (Grant No. 2007AA10Z401)

摘　　要：In this paper, we describe the synthesis of a novel copper ion hapten using the copper mercaptide of penicillenic acid （CMPA） derived from penicillin. Results from tests with immune rabbits indicate that： （i） A new antigen synthesized with CMPA has good stability and is safe for immunizing animals with no toxic phenomena being found in animal experiments; （ii） the immunogenic antigen （CMPA-BSA） can stimulate the immune system to produce specific antibodies with high titrations, up to 150000; and （iii） antibodies in antisera showed higher affinity to OVA-GSH-CuC1 than OVA-GSH, which indicates that the antibodies have specific affinity towards copper ions. These results confirm that the novel hapten and relevant antigen for copper ion have been successfully synthesized, giving progress towards an immunoassay for copper ions in environmental and food samples.In this paper, we describe the synthesis of a novel copper ion hapten using the copper mercaptide of penicillenic acid (CMPA) derived from penicillin. Results from tests with immune rabbits indicate that: (i) A new antigen synthesized with CMPA has good stability and is safe for immunizing animals with no toxic phenomena being found in animal experiments; (ii) the immunogenic antigen (CMPA-BSA) can stimulate the immune system to produce specific antibodies with high titrations, up to 150000; and (iii) antibodies in antisera showed higher affinity to OVA-GSH-CuCl than OVA-GSH, which indicates that the antibodies have specific affinity towards copper ions. These results confirm that the novel hapten and relevant antigen for copper ion have been successfully synthesized, giving progress towards an immunoassay for copper ions in environmental and food samples.

关 键 词：IMMUNOASSAY copper ion HAPTEN ENVIRONMENT food sample 

分 类 号：S854.43[农业科学—临床兽医学] TQ465.1[农业科学—兽医学]