圈养梅花鹿BDNF基因多态性与日常行为性状的关联分析  

The association of BDNF gene polymorphisms with normal behavior traits in house-hold sika deer(Cervus nippon)

作　　者：吕慎金[1] 杨燕[2] 魏万红[3] 

机构地区：[1]临沂大学生命科学学院,临沂276005 [2]山东省临沂市农业科学院,临沂276012 [3]扬州大学生命科学与技术学院,扬州225009

出　　处：《生态学报》2011年第17期4881-4888,共8页Acta Ecologica Sinica

基　　金：国家自然科学基金项目(31001027)

摘　　要：采用目标动物取样法(Focal Animal Sampling)和全事件记录方法(All-occurrence Recording),观察统计了江苏省扬州市平山堂养殖场及扬州市动物园共48只梅花鹿昼间日常行为节律。以脑源性神经生长因子(Brain-derived Neurotrophic Factor,BDNF)为目的基因,采用PCR-SSCP方法,研究梅花鹿行为性状与基因多态性之间的关联。结果表明,在P-4引物,AA与BB基因型在卧息行为上存在显著差异(P<0.05),在运动行为上存在极显著差异(P=0.001)。在P-5引物,CC/DD/CD基因型在观望行为上两两差异显著,并且CC和DD基因型在卧息与修饰行为均存在显著差异(P<0.05)。研究表明,BDNF基因多态性和动物日常行为性状存在一定相关。It is widely accepted that brain derived neurotrophic factor（BDNF） is involved in modulating behavioral performance in response to environmental conditions.The BDNF gene has been intensively studied;however,no studies have addressed the effect of BDNF gene polymorphisms on behavioral traits in sika deer（Cervus nippon）.This study investigated the relationship between single nucleotide polymorphisms（SNPs） in the BDNF gene and behavior traits of sika deer in a semi-housed environment.This study was performed on Ping-Shan-Tang farm（25 deer） and in Zhu-Yu-Wan Park（23 deer）,Yangzhou,Jiangsu province,China.The deer on Ping-Shan-Tang farm were housed in four 20×10 m paddocks with shelters of mantle.In Zhu-Yu-Wan Park,the deer roamed in a fenced paddock of grasses,shrubs and trees（120 m × 80 m）.All deer were marked by ear-notches and collar-tags for easy identification and were aged between 3 and 7 years.Observations were conducted at the same time each day between 08：30 a.m to 16：30 p.m and deer were sampled in a different order each day（using random numbers）.Each individual was studied for a mean（±S.E.） of（33.3±0.09） h and over a total of 50 weeks,1600 h were spent observing the deer.All data were obtained with focal animal sampling and all-occurrence recording.After all observations,blood samples（10 mL） were collected from the jugular vein using vacuum tubes with acid citrate dextrose anticoagulant and then stored at-20℃.Genomic DNA was extracted from whole blood using a standard phenol/chloroform protocol,dissolved in sterile water at a concentration of 100 ng/μL and then kept at-20℃ until use.PCR amplification and SSCP analysis of the BDNF gene were performed using six pairs of primers.Two pairs of primers（P-4 and P-5） amplified polymorphic fragments.P-4 yielded a 249 bp fragment and P-5 yielded a 224 bp fragment.Three genotypes were identified by SSCP for each of the fragments amplified by the primer pairs.All behavior trait data were analyzed by one way ANOVA

关 键 词：梅花鹿 BDNF多态性 PCR-SSCP 行为性状 

分 类 号：S825[农业科学—畜牧学]