基于聚吡咯/多壁碳纳米管复合膜的电化学DNA生物传感器研究  

DNA electrochemical biosensor based on polypyrrole/multiwalled carbon nanotubes composite film

作　　者：张玉雪[1] 牟靖男[2] 刘丽燕[2] 王茂清[2] 杜晓燕[2] 

机构地区：[1]哈尔滨医科大学公共卫生学院食品药品安全评价中心,黑龙江哈尔滨150081 [2]哈尔滨医科大学公共卫生学院营养与食品卫生学教研室,黑龙江哈尔滨150081

出　　处：《中华疾病控制杂志》2011年第9期745-748,共4页Chinese Journal of Disease Control & Prevention

基　　金：国家自然科学基金(30972488);黑龙江省教育厅科学技术研究项目(11541207)

摘　　要：目的利用电化学DNA生物传感器技术,建立一种简单快速的沙门氏菌检测方法。方法利用循环伏安法将新蒸单体吡咯和羧基多壁碳纳米管聚合到电极表面,形成均匀牢固、富含羧基的修饰膜。导电聚合物薄膜使电极获得了大的比表面积和优异的电子传递能力。在偶联剂1-乙基-(3-二甲基氨基丙基)碳化二亚胺盐酸盐(EDAC)的作用下,亲和素与电极表面的羧基发生共价反应,进而与生物素标记的DNA探针结合,使探针固定到电极表面。结果采用电化学阻抗法对目的基因进行检测,线性范围为1.0×10-9mol/L^1.0×10-7mol/L,检测下限为5.0×10-10mol/L。结论用聚吡咯/羧基多壁碳纳米管修饰玻碳电极,通过生物素-亲和素体系固定探针,制备一种电化学DNA生物传感器,可实现对沙门氏菌毒力基因invA的特异性基因片段的快速检测。Objective To build a simple and fast method for salmonella detection by the technology of electro- chemical DNA biosensor. Methods New distilled pyrrole and multiwalled nanotubes were polymerized onto the surface of carbon glass electrode by cyclic voltammetry to form a robust and well-distribute film, which contained carboxylic groups. Conductive polymer film combines the advantages of the fine conductivity of carbon nanotuhes and the large active surface area of polypyrrole. Avidin and carboxyl on the electrode surface were crosslinked by using 1-ethyl-3-（ 3-dimethy- laminopropyl） carbodiimide hydrochloride （EDAC）. Then, biotin-ssDNA was immobilized on the modified electrode sur- face via biotin-avidin system. Results The technique of A.C. impedance was applied to detect impedance （RET） signal of target DNA. The range of linearity was 1.0×10-9mol/L- 1.0×10^-7,ol/L,. The detection limit was 5.0×10^- 10mol/L. Conclusions An electrochemical DNA biosensor based on polypyrrole/multiwalled carbon nanotubes modi- fied electrode and biotin-avidin system fixed DNA probes could be developed for the fast detection of salmonella.

关 键 词：吡咯类 纳米管 碳 生物传感技术 

分 类 号：R113[医药卫生—公共卫生与预防医学] R115