时间分辨技术定量检测儿童乙肝病毒抗-HBs水平的研究  

Research on time resolved fluorescence immunoassay in quantitative detection of the anti-HBs of children's serum

作　　者：程邦宁[1,2] 李林[1,2] 金载璇[1,2] 郝家砚[1,2] 孙亮[1,2] 许莉莉[1,2] 张功[1,2] 

机构地区：[1]安徽医科大学儿科临床学院 [2]安徽省立儿童医院检验科,安徽合肥230051

出　　处：《中华疾病控制杂志》2011年第9期788-790,共3页Chinese Journal of Disease Control & Prevention

基　　金：安徽省卫生厅课题(09c198)

摘　　要：目的定量检测儿童体内乙型肝炎病毒（hepatitis B virus,HBV）血清标志物抗-HBs的含量;对儿童体内抗-HBs不同含量进行统计,分析其对HBV的免疫力。方法运用时间分辨荧光免疫技术对3 480例非HBV感染儿童进行HBV抗-HBs定量检测。结果抗-HBs含量在0~mIU/ml 817人（23.5%）、在10~100 mIU/ml 1 142人（32.8%）、〉100 mIU/ml 1 521人（43.7%）,不同性别间抗-HBs含量差异无统计学意义（Z=0.34,P=0.735）;不同年龄组之间抗-HBs含量差异有统计学意义（Z=3 634.00,P〈0.001）,且年龄组与抗-HBs含量呈现负相关（rs=-0.217,P〈0.001）。结论安徽省部分儿童对HBV的免疫力不容乐观,且随着年龄的增长,抗-HBs水平呈下降趋势,因此定期定量检测抗-HBs含量和加强乙肝疫苗预防接种非常必要。Objective To detect the anti-HBs of children＇ s serum quantitatively, so as to analyze their immunity to hepatitis B virus （HBV）. Methods Time resolved fluorescence immunoassay was used to measure the anti-HBs of 3 480 serum samples from the children of non-infective HBV. Results There were 817 cases in 0- mIU/ml about content of the anti-HBs （23.5%）, 1 142 cases in 10-100 mIU/ml （32.8%）, 1 521 cases 〉100 mIU/ml （43.7%）. There was no statistical difference between deferent sex of the anti-HBs （Z = 0.34, P = 0. 735）. There was statistical dif- ference between different age groups （Z =3 634.00, P 〈 0.001 ）, the group age and the content of the anti-HBs was neg- atively correlated （ rs = - 0. 217, P 〈 0. 001）. Conclusions The part of children, immunity to HBV is the worst scenario, and along with age growing, the content of the anti-HBs is downtrending, therefore, it is very necessary to detect the anti-HBs and vaccinate hepatitis B vaccine at regular intervals and quantitatively.

关 键 词：荧光免疫测定 肝炎病毒 乙型 抗体 

分 类 号：R512.62[医药卫生—内科学]