基于药效学试验的黄连解毒汤大孔树脂精制工艺筛选  

Study on Refining Process Screening of Huanglian Jiedu Decoction by Macroporous Resin Based on Pharmacodynamical Test

作　　者：朱华旭[1] 李欢[1] 顾晓莹[1] 钱智磊[1] 唐于平[1] 

机构地区：[1]南京中医药大学中药复方分离工程重点实验室江苏省方剂重点实验室,南京210029

出　　处：《中国实验方剂学杂志》2011年第18期1-5,共5页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30873450);江苏省教育厅‘青蓝工程’资助项目

摘　　要：目的:研究比较黄连解毒汤水提取液体系经大孔树脂精制后的药理活性及化学组成变化,探讨中药复方树脂精制工艺的优选原则,为中药复方的精制分离提供实验依据。方法:选用目前市售的10种不同极性的聚苯乙烯型大孔树脂对黄连解毒汤全方水提取液体系进行精制,采用鼠性肾上腺髓质嗜铬细胞瘤PC-12细胞活性跟踪筛选,MTT法测定全方水提取物及各大孔树脂精制物对低糖DMEM和氯化钴诱导的PC-12细胞缺糖缺氧损伤的影响。以RP-HPLC确定全方水提取物及各大孔树脂精制物中6种指标性成分———小檗碱、巴马汀、黄芩苷、黄芩素、汉黄芩素、栀子苷的含量。结果:经过X-5型大孔树脂精制后的黄连解毒汤水提取物能更好的保留原有复方各成分的比例,且药效作用与黄连解毒汤原方水提取液保持一致。结论:该研究为黄连解毒汤的临床用药剂型改进和实现工业化生产奠定了实验基础。Objective：To compare pharmacological activity and chemical composition changes of water extract from Huanglian Jiedu Decoction（HLJDT） after refined by 10 different Macroporous Resins.Investigated optimization principle of refining process by resin from traditional Chinese medicine compound,provided basis to refining and separation of traditional Chinese medicine compound prescription.Method：Ten different polarity type of polystyrene Macroporous Resins were selected to refine the water extract from HLJDT.Took activity tracking filter of rat adrenal medulla pheochromocytoma PC-12 cell,determined the effect of water extract and refined material to low-sugar of DMEM and the oxygen-glucose deprivation injury of cobalt chloride-induced PC-12cells by MTT.The content of six index ingredients（berberine,palmatine,baicalin,baicalein,wogonin and geniposide） were detected by RP-HPLC.Result：The results show：the refined material of water extract by X-5-type Macroporous Resin was better able to retain the existing ratio of each component in HLJDT,and the pharmacodynamic effects consistent with HLJDT.Conclusion：This study settled experimental basis to clinical improved formulations and of industrial production of HLJDT.

关 键 词：黄连解毒汤 大孔树脂 精制工艺 药效学筛选 

分 类 号：R283.6[医药卫生—中药学]