大承气汤中大黄酸在大鼠体内的药动学研究  

Pharmacokinetics of Rhein in Dachengqi Decoction in Rats

作　　者：韩刚[1] 肖倩[1] 索炜[1] 刘莉[1] 康欣[1] 王彦雪[1] 

机构地区：[1]河北联合大学药学院,河北唐山063000

出　　处：《中国实验方剂学杂志》2011年第18期137-140,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究大鼠灌胃给予大黄及大承气汤后,大黄酸在大鼠体内药动学过程。方法:大鼠分别灌胃给予大黄及大承气汤,高效液相色谱法测定大黄酸经时血药浓度变化。色谱柱为Diomansil C18(4.6 mm×150 mm,5μm),流动相甲醇-水-冰醋酸(77∶22∶1),检测波长428 nm,流速1.0 mL.min-1。血药浓度-时间数据用3P97药动学软件处理。结果:大黄酸血药浓度在1.0～15 mg.L-1线性关系良好。大鼠灌胃给予大黄及大承气汤后大黄酸血浆浓度-时间曲线均符合二房室模型,大黄及大承气汤的主要药动学参数AUC和Cmax均存在显著性差异(P<0.05)。结论:大承气汤中大黄与其他成分配伍后,使大黄酸在大鼠体内的血药浓度降低。Objective：To study the pharmacokinetics of rhein rats were given of Radix et Rhizoma Rhei and Dachengqi Decoction.Method：Radix et Rhizoma Rhei group were given a single dose of Radix et Rhizoma Rhei and Dachengqi Decoction group were given Dachengqi Decoction.The plasma concentration of rhein was measured by HPLC.Diomansil C18（4.6 mm ×150 mm,5 μm）was used as analytical column.The mobile phase consisted of methanol-water-acetic acid（77：22：1）,and the wavelength of detection was set at 428 nm.Plasma concentration-time data were analysted with the 3P97 pharmacokinetic software.Result：The linear ranges of rhein were from 1.0 to 15 mg·L-1.The concentration-time curves of rhein were fitted to the two compartment models in rats.The main pharmacokinetic parameters of the concentration-time curves and the maximum plasma concentration were significant difference between the Radix et Rhizoma Rhei groups and Dachengqi Decoction group after administrated the same dosage of rhein in rats（P0.05）.Conclusion：It was decreased the concentration of rhein in plasma when Radix et Rhizoma Rhei compatibility with other chinese medicine in Dachengqi Decoction.

关 键 词：大承气汤 大黄 大黄酸 药动学 HPLC 

分 类 号：R285.5[医药卫生—中药学]