解毒化瘀药含药血清对白血病HL60/ADR耐药细胞NF-κB信号靶向干预研究  

Influence of Drug-containing Serum Prepared for Jiedu Huayu Medicine on Expression of NF-κB Pathway in Leukemia HL60/ADR Cell

作　　者：马武开[1] 姚血明[1] 王莹[1] 黄颖[1] 黄礼明[1] 

机构地区：[1]贵阳中医学院第二附属医院,贵阳550003

出　　处：《中国实验方剂学杂志》2011年第18期154-157,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(30860353);贵州省高层次人才科研特助项目

摘　　要：目的:探讨解毒化瘀药含药血清对白血病HL60/ADR细胞核周因子κB(NF-κB)信号基因表达的影响。方法:将HL60/ADR耐药细胞分为牛血清组、兔血清组、含药血清高、中、低剂量处理组和干扰素对照组,用Western blot法检测各组细胞NF-κB/p65,NF-κB/p50,IκBα的表达情况。结果:流式细胞仪检测结果显示非细胞毒作用的解毒化瘀药血清能够增加HL60/ADR细胞内柔红霉素的浓度。HL60/ADR耐药细胞NF-κB信号基因呈高表达,HL60敏感细胞未见表达,加用不同浓度解毒化瘀含药血清处理后,p65,p50和IKBa的表达均有不同程度下降,以高、中剂量组对p65的下调作用更明显。结论:解毒化瘀药对HL60/ADR细胞的耐药逆转作用是通过阻断NF-κB信号通路实现的。Objective：To investigate the influence of drug-containing serum prepared for Chinese herbal medicine of Jiedu Huayu on the expression of nuclearfactor-κappaB（NF-κB）of leukemia HL60/ADR cell.Method：Western blot was applied for examination the expression of NF-κB/p65,NF-κB/p50,IκBα after HL60/ADR cell receiving the treatment of herbal serum of Jiedu Huayu.Result：The expression of NF-κB/p65,NF-κB/p50,IκBα after HL60/ADR cell was decreased by herbal serum of Jiedu Huayu.Conclusion：Jiedu Huayu Chinese herbal serum can reverse multidrug resistance in leukemia cell by blocking the expression of NF-κB pathway in leukemia HL60/ADR cell.

关 键 词：白血病 多药耐药 HL60/ADR细胞 NF-κB信号 解毒化瘀药 

分 类 号：R285.5[医药卫生—中药学]