参芪扶正注射液对化疗后免疫抑制的减毒作用  

The Attenuation of Shenqi Fuzheng Injection Treatment on Immunosuppression Caused by Chemotherapeutics

作　　者：史晓光[1] 丁治国[1] 张林[1] 

机构地区：[1]北京中医药大学东直门医院普外科,北京100700

出　　处：《中国实验方剂学杂志》2011年第18期158-160,共3页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：国家自然科学基金项目(81001524)

摘　　要：目的:探讨参芪扶正注射液含药血清对化疗药物所造成免疫抑制的减毒作用。方法:选用Wistar雄性大鼠20只,随机分为含药血清组和空白血清组。含药血清组按40 g.kg-1.d-1的剂量尾静脉注射参芪扶正浓缩液,每日给药2次,连续给药5次。空白血清组给予生理盐水代替参芪扶正浓缩液,余相同。2组大鼠于末次给药后1 h后自腹主动脉采血,分离血清。研究设药物组和对照组。药物组分别用5-氟尿嘧啶(5-Fu)注射液(50 mg.L-1)、顺铂注射液(6.25 mg.L-1)、参芪扶正注射液含药血清作用于小鼠巨噬细胞系RAW264.7细胞;对照组用空白血清作用于RAW264.7细胞。利用酶标仪对作用后的RAW264.7细胞进行检测,观察5-Fu注射液、顺铂注射液、含药血清对RAW264.7细胞增殖的影响。结果:单用参芪扶正注射液组与空白对照组相比能明显促进RAW264.7细胞增殖(P<0.01)。5-Fu、顺铂注射液联合含药血清后与单用5-Fu、顺铂注射液相比,对于RAW264.7细胞增殖的抑制明显降低,其差异皆有显著性(P<0.01)。结论:参芪扶正注射液在体外环境下可促进巨噬细胞的增殖,改善化疗药物所造成的免疫抑制,对不同化疗药物的减毒趋势相同而减毒强度不同。Objective：To explore attenuation that serum containing drug of Shenqi Fuzheng injection treatment on immunosuppression caused by chemotherapeutics.Method：Twenty male Wistar rates are divided into drug serum group and control group.Drug serum group is injected through caudal vein with 40 g·kg-1·d-1 Shenqi Fuzheng injection twice a day and five times in total.Control group are injected with 0.9%NaCl solution instead of Shenqi Fuzheng injection and others are the same.All the rates＇ blood are collected from abdominal aorta and then serum are Separated.Researches are divided into drug group and control group.In drug group,RAW264.7 cells are treated with Fluorouracil injection（50 mg·L-1）,Cisplatin injection（6.25 mg·L-1）,drug serum and in control group no-drug serum is used instead of drug.Treated RAW264.7 cells are detected with microplate reader.Observe effect on Fluorouracil injection,Cisplatin injection,serum containing drug of Shenqi Fuzheng injection on cell multiplication of RAW264.7 cells.Result：The serum containing drug of Shenqi Fuzheng injection can promote RAW264.7 cells multiplication（P0.01）.The serum containing drug of Shenqi Fuzheng injection can produce attenuation on immunosuppression caused by Fluorouracil injection and Cisplatin injection（P0.01）.Conclusions：In vitro environment Shenqi Fuzheng injection can promote RAW264.7 cells multiplication,and improve immunosuppression caused by chemotherapeutics.It can induce same trend and different intension of attenuation on different types of chemotherapy drugs.

关 键 词：化疗 参芪扶正注射液 RAW264.7细胞 

分 类 号：R285.5[医药卫生—中药学]