虎杖提取物对实验性脑梗死大鼠脑组织炎性因子的影响  

Protective Effects of Extract from Polygonum cuspidatum on Focal Cerebral Ischemia-reperfusion Injury in Rats

作　　者：李晓莉[1] 卢志刚[1,2] 陈梅[1] 蔡志平[3] 

机构地区：[1]荆楚理工学院医学院,湖北荆门448000 [2]荆楚理工学院附属荆门市第一人民医院,湖北荆门448000 [3]河北医科大学,石家庄050017

出　　处：《中国实验方剂学杂志》2011年第18期226-229,共4页Chinese Journal of Experimental Traditional Medical Formulae

摘　　要：目的:研究虎杖提取物对大鼠局灶性脑缺血再灌注炎性损伤的保护作用。方法:将SD大鼠随机分为6组ig给药,假手术组和模型组给予等量生理盐水;尼莫地平组按1 mg.kg-1给予尼莫地平;虎杖提取物低、中、高剂量组分别按5,10,20 mg.kg-1给予虎杖提取物。给药7 d后制作大鼠大脑中动脉闭塞模型,观察大鼠行为学改变;取大脑并用氯化四唑染色后测定梗死百分比和含水量;进行大脑组织学检查,制作脑匀浆测定白介素(IL)-1β,IL-6,IL-8和一氧化氮(NO)的含量。结果:虎杖提取物高、中剂量组的行为学评分分别为(2.6±0.9),(2.2±1.1)分;梗死率分别为(37.6±1.9)%,(30.2±4.2)%;脑含水量分别为(78.1±5.6)%,(71.2±3.9)%,均较模型组显著降低(P<0.01);IL-1β分别为(0.64±0.28),(0.65±0.31)μg.L-1;IL-6分别为(7.2±2.7)ng.L-1,(8.8±2.2)ng.L-1,IL-8分别为(12.6±2.5)μg.L-1,(15.2±2.1)μg.L-1和NO分别为(2.9±1.3),(3.2±1.1)mmol.L-1,较模型组均明显降低(P<0.05,P<0.01)。结论:虎杖提取物对脑缺血再灌注炎性损伤有一定的保护作用。Objective：To investigate the protective effects of extract from Polygonum cuspidatum on focal cerebral ischemia-reperfusion（I/R） injury in rats.Method：SD rats were randomly divided into 6 groups：sham operated group,model group,nimodipine group（1 mg·kg-1） and 3 groups treated with extract from P.cuspidatum（5,10,20 mg·kg-1）.The rat model of middle cerebral artery occlusion（MCAO） was induced to observe the changes in cerebral function score,infarction area,water content,and histological modifications in rat brain.The levels of Interleukin-1β（IL-1β）,interleukin-6（IL-6）,interleukin-8（IL-8）,nitric oxide（NO） were also measured.Result：Extract from P.cuspidatum at different doses could obviously decrease the cerebral function score,infarction area and water content.It also alleviated the pathological injury of focal cerebral I/R injury in rats.For extract from P.cuspidatum groups,IL-1β,IL-6,IL-8 and NO in brain tissue were significantly decreased.Conclusion：Extract from P.cuspidatum has protective effects on cerebral I/R injury.

关 键 词：虎杖提取物 脑缺血 再灌注 保护作用 

分 类 号：R285.5[医药卫生—中药学]