半夏、掌叶半夏及禹白附凝集素蛋白的刺激性研究  

Irritability of Agglutinin from Pinellia ternata Breit,Pinellia pedtaisecta Schott and Typhonium giganteum Engl.

作　　者：刘先琼[1,2] 吴皓[1] 郁红礼[1] 潘耀宗[1] 

机构地区：[1]南京中医药大学药学院,南京210046 [2]湖北中医药大学药学院,武汉430065

出　　处：《中国实验方剂学杂志》2011年第18期244-247,共4页Chinese Journal of Experimental Traditional Medical Formulae

基　　金：江苏省2011年度研究生培养创新工程;国家自然基金项目(NO.30973939);国家"十一五"科技支撑计划课题(2006BAI09-10)

摘　　要：目的:研究半夏凝集素(Pinellia ternata agglutinin,PTA),掌叶半夏凝集素(Pinellia pedtaisecta agglutinin,PPA),禹白附凝集素(Typhonium giganteum agglutinin,TGA)的刺激性作用。方法:SD雄性大鼠随机分为空白组,PTA,PPA及TGA高中低剂量组(300,200,100 mg.L-1),ip给药,4 h后处死大鼠,ip生理盐水,取腹腔液测中性粒细胞迁移数目,测定腹腔液中总蛋白,一氧化氮(NO),前列腺素E2(PGE2)的含量;家兔随机分为空白组,半夏,掌叶半夏及禹白附的凝集素组及毒针晶组,半夏,掌叶半夏及禹白附的针晶加其凝集素组,观察家兔眼结膜充血,水肿及分泌物,考察PTA,PPA,TGA的刺激性作用。结果:大鼠ip PTA,PPA,TGA后,与空白组腹腔液中性粒细胞迁移数目(633.33±233.81)×106/L比较,100 mg.L-1组PTA(1 083.33±116.9)×106/L,PPA(1 033.3±150.55)×106/L及TGA(1 133.33±103.28)×106/L均具极显著性差异(P<0.01),均可引起严重的中性粒细胞向腹腔迁移,且浓度升高,作用增强;大鼠腹腔液的总蛋白,NO及PGE2的含量显著升高,均可加重针晶刺激家兔眼结膜强烈水肿。结论:天南星科中具有刺激性毒性作用的半夏,掌叶半夏及禹白附所含凝集素蛋白具有刺激性作用,是其毒性刺激性成分。Objective：To study the irritability of agglutinin from P.ternate Breit,P.pedtaisecta Schott and Typhonium giganteum Engl.Method：Male SD rats were designed to control group,low dose group（100 mg·L-1）,medium dose group（200 mg·L-1） and high dose group（300 mg·L-1） randomly.The control group received the same volume of saline in substitution of the lectin solution.Four hours later,rats were sacrificed and peritoneal cells harvested by washing each peritoneal cavity with saline.The irritation effect of P.ternata agglutinin（PTA）,P.pedtaisecta agglutinin（PPA） and T.giganteum agglutinin（TGA） was observed in model of rat neutrophil migration in vivo by measurement of the number of neutrophils and the content of protein,NO and PGE2 of peritoneal fluid.Rabbits were designed to control group,Raphides of P.ternate group,P.pedtaisecta group and T.giganteum group,PTA and Raphide of P.ternate group,PPA and Raphide of P.pedtaisecta group,TGA and Raphide of T.giganteum group.Conjunctival congestion of rabbit was observed.Result：After injection of PTA,PPA and TGA,compared with control group（633.33 ± 233.81）×106/L the number of neutrophil migration in the low-dose group of PTA（1 083.33±116.9）×106/L,PPA（1 033.3±150.55）×106/L and TGA（1 133.33±103.28）×106/L was significant increased（P0.01）.Neutrophil migration was induced and the content of protein,NO and PGE2 was elevated by PTA,PPA,TGA.PTA,PPA,TGA that showed obvious irritation effects on conjunctival congestion of rabbit.Conclusion：Agglutinin is the irritative component from P.ternata,P.pedtaisecta and Typhonium giganteum.

关 键 词：半夏凝集素 掌叶半夏凝集素 禹白附凝集素 刺激性作用 

分 类 号：R285.5[医药卫生—中药学]