耐碳青霉烯鲍曼不动杆菌OXA酶基因研究  

Study on oxacillinase encoding gene in carbapenem-resistant Acinetobacter baumannii

作　　者：杨培洪[1,2] 凌保东[1] 

机构地区：[1]川北医学院药物研究所药理学教研室,南充637007 [2]彭州市人民医院药剂科,成都611930

出　　处：《中国抗生素杂志》2011年第9期703-707,共5页Chinese Journal of Antibiotics

基　　金：国家自然科学基金(No.30973662)

摘　　要：目的检测鲍曼不动杆菌对β-内酰胺类抗生素的耐药性，了解耐碳青霉烯鲍曼不动杆菌OXA酶基因、插入序列和I类整合酶基因的分布。方法采用琼脂二倍稀释法检测90株鲍曼不动杆菌对20种β-内酰胺类抗菌药物的最低抑菌浓度，常规PCR检测耐碳青霉烯鲍曼不动杆菌的4种OXA酶基因、插入序列和I类整合酶基因。结果90株鲍曼不动杆菌对20种β-内酰胺类抗菌药物的耐药性为5．7％～94．2％；21株耐碳青霉烯鲍曼不动杆菌中，8株OXA-23阳性，且/SAba1同为阳性，4株OXA．51阳性，1株I类整合酶基因阳性。结论临床分离的鲍曼不动杆菌对β-内酰胺类抗生素耐药形势严峻，耐碳青霉烯鲍曼不动杆菌OXA酶基因和ISAba，检出率均很高。Objective To test the sensitivity of Acinetobacter baumannii to β-lactam antibiotics and detect oxacillinase encoding genes, 1SAbal, ISAba4 and class 1 integtrase gene（intI1） in carbapenem-resistant Acinetobacter baumannii. Methods A total of 90 non-duplicate clinical isolates of Acinetobacter baumannii were recovered and antimicrobial susceptibility was determined by two-fold disk diffusion and agar dilution. The antimicrobial resistance related genes including oxacillinase encoding genes, ISAbal, ISAba4 and class 1 integtrase gene（intI1） in the carbapenem antibiotics resistant strains were detected by regular polymerase chain reaction（PCR）. Results The resistant rates of 90 strains ofAcinetobacter baumannii were 5.7%-94.2% to 20 β-lactam antibiotics. Of the 21 strains of carbapenem-resistant Acinetobacter baumannii, 8 isolates were shown positive to OXA-23, and the blaoxA.23 gene was found to coexist with ISAbal gene in those isolates, 4 isolates were positive to OXA-51, and 1 isolates was positive to class 1 integtrase gene. Conclusion The clinical isolates of Acinetobacter baumannii in Northeastern Sichuan are extensively resistant to most commonly used β-lactam antimicrobial agents. The detection rates of oxacillinase encoding and ISAbal genes are very high in carbapenem-resistant Acinetobacter baumannii.

关 键 词：鲍曼不动杆菌 碳青霉烯酶 苯唑西林酶 

分 类 号：R378[医药卫生—病原生物学]