天花粉蛋白对卵巢癌HO8910细胞凋亡及Bax、Bcl-2基因表达的影响  

Trichosanthin on Ovarian Cancer HO8910 Cell Apoptosis and Bax,Bcl-2 gene in Expression

作　　者：候贺佳[1] 张爽[1] 

机构地区：[1]辽宁医学院附属第一医院妇产科,辽宁锦州121000

出　　处：《辽宁中医杂志》2011年第9期1729-1730,共2页Liaoning Journal of Traditional Chinese Medicine

摘　　要：目的:探讨天花粉蛋白(TCS)对卵巢癌细胞株HO8910增殖效应并探讨其机制。方法:采用MTT法检测不同浓度(20、40、80μmol/L)对细胞HO8910增殖的影响;通过流式细胞仪观察细胞(20μmol/L)凋亡过程中细胞周期的变化;Western Blot检测不同浓度(20、40、80μmol/L)72hTCS对HO8910凋亡相关蛋白Bax、Bcl-2的表达。结果:随着TCS浓度的升高,作用时间的延长,TCS对HO8910生长抑制率逐渐增高(P<0.05),呈一定的时间,剂量依赖性。流式细胞仪检测随着天花粉蛋白浓度的增加,Bax基因的表达增加而Bcl-2的表达减少。结论:TCS对卵巢癌细胞HO8910细胞增殖抑制作用,该作用可能与其下调Bcl-2及上调Bax蛋白表达有关。Objective ：To investigate the effect of trichosanthin on ovarian cancer cell line HO8910 inhibiting the proliferation effect and apoptosis - inducing effects and explore its apoptosis - related genes Bax and Bcl - 2 expression. Methods ： MTT assay of drugs on cells in vitro ;flow cytometry cell apoptosis in the cell cycle changes. Western Blot Detection of apoptosis -related proteins Bax, Bcl - 2 expression. Results ： With the drug concentration increased, the role of the time extension of triehosanthin on HO8910 cell growth inhibition rate was gradually increased ;under the effect of triehosanthin, HO8910 cells apoptotic morphological changed and apoptotic cells histological changed. Flow cytometry histogram also appears characteristic of the sub - diploid peak, with the increase in the concentration of trichosanthin, Bax gene expression increases the expression of Bcl - 2 reduction. Conclusion：Trichosanthin can induce HO8910 ovarian cancer cell apoptosis. Bcl -2 protein in the reduction of Bax protein expression increasing apoptosis in ovarian cancer cells may be one of the mechanisms.

关 键 词：卵巢肿瘤 天花粉蛋白 HO8910细胞 BCL-2 BAX 

分 类 号：R285.5[医药卫生—中药学]