鸡传染性支气管炎病毒0801株的分离鉴定及S1基因序列分析  

Isolation and Sequence Analysis of S1 Gene of Avian Infectious Bronchitis Virus 0801 strain

作　　者：刘巍[1] 孔义波 张兴晓 柴同杰[1] 

机构地区：[1]山东农业大学动物医学院,山东泰安271018 [2]山东省动物用生物制剂工程技术中心,山东烟台264003

出　　处：《动物医学进展》2011年第8期1-6,共6页Progress In Veterinary Medicine

基　　金：国家自然科学基金项目(31072189)

摘　　要：2008年1月,河北沧州某产蛋鸡场鸡群出现呼吸道症状,少数鸡只出现死亡,剖检发现鸡只肾脏肿大,呈"花斑肾"样,收集患病鸡只肾脏、气管等组织,进行病毒分离培养。经新城疫病毒干扰试验、血凝试验、动物回归试验、鸡胚交叉中和试验及分子生物学鉴定,证实分离到一株鸡传染性支气管炎病毒,并命名为0801;鸡胚交叉中和试验结果表明,0801株与疫苗Ma5株中和指数最高,为同一血清亚型;对其S1基因进行克隆、序列测定与分析,表明0801株的S1基因全长1 620 bp,核苷酸序列与Massachusetts型疫苗株相似性在75%左右,氨基酸牙似性亦在75%左右。In the January of 2008, respiratory signs were observed in the chicks of a layer farm in Cangzhou, Hebei province. Necropsy of the dead chicks revealed that the kindey was swollen and distended with uric acid crystals. Kidneys and tracheas were collected for virus isolation. An IBV virus was identified by viral interference test, HA test, animal regressive research, cross-neutralization and RT-PCR. The virus was designated 0801 strain. The date of cross-neutralization indicated that the 0801 strain and Ma5 vaccine strain belong to the same serotype. The S1 gene of 0801 strain was amplified, cloned, sequenced and analyzed. The results showed that S1 gene was composed of 1620 nucleotides, homologies of nucleotide and amino acid sequences of S1 gene between 0801 strain and Massachusetts-type vaccine strains was both about 75%.

关 键 词：传染性支气管炎病毒 交叉中和试验 S1基因 序列分析 

分 类 号：S852.659.6[农业科学—基础兽医学] Q789[农业科学—兽医学]