铁线莲属8种药用植物ITS序列分析  

ITS sequence analysis of eight medicinal plants in Clematis L.

作　　者：蒋明[1] 周英巧[1] 李嵘嵘[2] 

机构地区：[1]台州学院生命科学学院,浙江临海317000 [2]台州学院医药化工学院,浙江临海317000

出　　处：《中草药》2011年第9期1802-1806,共5页Chinese Traditional and Herbal Drugs

基　　金：浙江省新苗人才计划项目(2009R428012)

摘　　要：目的通过测定并比较柱果铁线莲、单叶铁线莲、威灵仙、山木通、毛蕊铁线莲、转子莲、女萎和天台铁线莲的ITS序列,为铁线莲属药用植物的分子鉴定提供依据。方法利用PCR法从叶片基因组DNA中扩增ITS,借助Clustal X、MEGA、DNASTAR等软件比较和分析ITS的序列特征。结果铁线莲属8种药用植物ITS1与ITS2之间的长度为534～561 bp,变异位点50个,信息位点22个;天台铁线莲与转子莲的遗传距离最小,与女萎遗传距离最大,亲缘关系最远。序列已提交至NCBI数据库,登录号为JF714638-JF714645。结论获得铁线莲属8种药用植物的ITS序列,为分子鉴定奠定了基础。Objective To provide molecular evidence for identification of medicinal plants in Clematis L.,sequencing and comparison of ITS from C.uncinata,C.henryi,C.chinensis,C.finetiana,C.lasiandra,C.patens,C.apiifolia,and C.patens ssp.tientaiensis were performed.Methods ITS sequences were amplified from leaf genomic DNA by PCR.Sequence features were compared and analyzed using Clustal X,MEGA,and DNASTAR softwares.Results Between ITS1 and ITS2 of the eight medicinal plants in Clematis L.,the length of ITS region varied from 534-561 bp with 50 variable sites and 22 parsimony information sites.The smallest genetic distance was observed between C.patens ssp.tientaiensis and C.patens,and the largest existed between C.patens ssp.tientaiensis and C.apiifolia revealing their farest genetic relationship.Sequences were submitted to NCBI database with the registry numbers of JF714638-JF714645.Conclusion ITS sequences of the eight medicinal plants in Clematis L.are obtained,which could provide a foundation for molecular identification.

关 键 词：铁线莲属 药用植物 ITS序列 分子鉴定 PCR法 

分 类 号：R287.7[医药卫生—中药学]