尿激酶对肾间质纤维化大鼠肾组织Snail表达的影响及其机制  

Impact of Urokinase on Expression of Snail in Rats with Renal Interstition Fibrosis and Possible Mechanisms

作　　者：罗正茂[1] 刘宏[1] 何凤[1] 王寅[1] 丁海燕 童俊容[1] 

机构地区：[1]广州军区广州总医院肾脏内科 [2]海南省三亚市92155部队卫生队

出　　处：《中国全科医学》2011年第27期3123-3126,共4页Chinese General Practice

基　　金：广东省自然科学基金(8151001002000006);广东省医学科研基金(B2008149)

摘　　要：目的探讨尿激酶(UPA)对环孢素A(CSA)诱导的肾病大鼠肾组织炎症反应及Snail的影响。方法采用CSA制模,大鼠分为对照组、模型组(CSA组)和尿激酶组(UPA组)。采用Masson染色观察肾间质改变,ELISA法检测肾组织TNF-α水平,免疫组化SP法检测Snail蛋白的表达及分布,RT-PCR检测Snail mRNA的表达水平。结果 (1)肾脏病理改变:CSA组肾组织胶原沉积显著加重,UPA组较CSA组胶原沉积显著减弱(P<0.05);(2)肾组织TNF-α水平:CSA组TNF-α水平显著升高,UPA组TNF-α水平显著低于CSA组(P<0.05);(3)肾组织Snail蛋白表达:CSA组Snail蛋白表达较对照组显著增加(P<0.05),且多位于细胞核内,UPA组Snail蛋白表达显著低于CSA组(P<0.05);(4)肾组织Snail mRNA表达:与对照组相比,CSA组Snail mRNA表达量显著升高(P<0.05),UPA组Snail mRNA表达量显著低于CSA组(P<0.05)。结论 UPA能改善CSA诱导的间质炎症反应和抑制Snail表达。Objective To explore the impact of Urokinase（UPA） on cyclosporin A induced inflammation and the expression of snail in rats.Methods CSA gavage was applied for establishment of the disease model and the animals were randomly divided into three groups：control group,CSA group and UPA group.Renal interstitial fibrosis was graded according to Masson staining.Levels of inflammatory factor TNF-were tested using ELISA.Protein expression and distribution of Snail1 were determined by immunohistochemistry and mRNA expression of Snail1 was examined by RT-PCR.Results（1） Collagen deposition was increased in the CSA group compared with the control group but the UPA group showed a significantly reduced level of collagen deposition than the CSA group（P0.05）.（2） TNF-α levels were significantly higher in the CSA group than in the control group and significantly lower in the UPA group than in the CSA group（P0.05）.（3）Snail protein levels were significantly higher in the CSA group,which mostly located in the nuclei,than in the control group（P0.05） and significantly lower in the UPA group than in the CSA group（P0.05）.（4）Snail mRNA levels were significantly higher in the CSA group than in the control group（P0.05） and significantly lower in the UPA group than in the CSA group（P0.05）.Conclusion Urokinase alleviates renal fibrosis in in rats with cyclosporin A induced nephropathy and decreases the expression levels of snail.

关 键 词：肾间质纤维化 尿激酶 环孢素A 肿瘤坏死因子-Α SNAIL 

分 类 号：R692.9[医药卫生—泌尿科学]