HPLC测定酒精性肝病大鼠血浆中PDGF的含量  

Determination of PDGF in Alcoholic Hepatitis Rats Plasma by HPLC

作　　者：万永臻[1] 张玉萍[1] 方步武[2] 

机构地区：[1]天津中医药大学,天津300193 [2]天津医科大学,天津300203

出　　处：《现代中药研究与实践》2011年第5期46-48,共3页Research and Practice on Chinese Medicines

基　　金：天津市应用基础研究计划重点项目(项目编号06YFJZJC02900)

摘　　要：目的建立酒精性肝病大鼠血浆中PDGF含量的HPLC测定方法。方法色谱柱为Hypersil C18柱(250 mm×4.6 mm,5μm),流动相为乙腈-水,梯度洗脱(5%～ 65%,40 min),流速1.0mL/min,柱温30℃,检测波长210 nm,进样量10μL。结果 PDGF线性范围浓度在0.01～0.30μg/mL时与峰面积呈良好的线性关系(r=0.999 3),测定方法回收率78.6%,RSD<15%。结论 该方法简便、快捷,可用于酒精性肝病大鼠血浆中PDGF的含量测定。Objective To establish an HPLC method for determination of platelet-derived growth factor （PDGF） level in rats plasma. Methods The HPLC analysis was performed on the Hypersil C18 column （250 mm ×4.6 mm, 5 μm） at temperature 30℃.The mobile phase consisted of acetonitrile and water, using gradient elution （5% - 65%, 40 min） with the flow rate 1.0 mL·min^-1. The detection wavelength was 210 nm. Results The standard curve was linear in the range of 0.01-0.30μg·mL^-1 （r =0.999 3）. The method recovery was 78.6%, and the intra-day and inter-day precisions were less than 15%. Conclusion The method is simple,convenient and rapid, which could be used to determine the PDGF in alcoholic hepatitis rats plasma.

关 键 词：PDGF HPLC 含量测定 

分 类 号：R285.5[医药卫生—中药学]