菊花开花持续期的QTL定位  

QTL Mapping for Flowering Duration Stage of Chrysanthemum

作　　者：张飞[1,2] 陈发棣[1] 房伟民[1] 陈素梅[1] 张鲜艳[1] 尹冬梅[1] 

机构地区：[1]南京农业大学园艺学院,南京210095 [2]浙江省农业科学院花卉研究开发中心,杭州311202

出　　处：《园艺学报》2011年第8期1557-1564,共8页Acta Horticulturae Sinica

基　　金：国家自然科学基金项目(30871724);公益性行业(农业)科研专项(200903020);上海市科技兴农重点攻关项目[沪农科攻字(2009)第3-3号]

摘　　要：以菊花‘雨花落英’ב奥运含笑’F1群体为材料,调查了2008—2009两个年度开花持续期的分离表现,并基于菊花SRAP遗传图对其进行QTL定位分析。基于单年数据,运用Win QTL Cartographerv2.5软件及复合区间作图法在2008年共检测到3个控制开花持续期的加性QTL,主要分布在秋菊‘雨花落英’遗传图的Y20和Y42连锁群以及夏菊‘奥运含笑’遗传图的A14连锁群上,LOD值介于2.65~3.44之间,单个QTL对开花持续期表型变异的贡献率为6.54%~11.58%,而在2009年未检测到QTL的存在。运用QTL Networkv2.2软件对2008和2009两年的表型数据进行联合分析,共检测到两对上位性QTL,在贡献率和效应值上与加性QTL相当,同时上位性QTL与环境之间具有互作效应。In this study,flowering duration stage of chrysanthemum were evaluated in 2008 and 2009,and QTL analysis was undertaken for this trait based on the two-year phenotypic data in 142 F1 mapping population derived from a cross between chrysanthemum cultivars of＇Yuhualuoying＇and ＇Aoyunhanxiao＇and their SRAP-based genetic linkage maps presently published.The QTL analysis based on the single-year data of 2008 and 2009 with Win QTL Cartographer v2.5 and composite interval mapping method indicated that 3 additive QTL were detected in 2008 but none in 2009.The three additive QTL scattered on linkage group Y20 and A42 of＇Yuhualuoying＇map and A14 of＇Aoyunhanxiao＇map,with a LOD value of 2.65-3.44 and contribution ratio for phenotypic variation ranging from 6.54% to 11.58% for each QTL.The QTL joint analysis based on the two-year phenotypic data of 2008 and 2009 with QTLNetwork v2.2 suggested that 2 additive-additive epsitatic QTL were mapped,with the similar contribution ratio and effect value to additive QTL detected by Win QTL Cartographer v2.5.In addition,there was interaction between the 2 epistatic QTL and environment.

关 键 词：菊花 F_1群体 开花持续期 QTL定位 

分 类 号：S682.11[农业科学—观赏园艺]