外伤性癫痫患者癫痫灶中GABABR1和GluR1的表达  

The expression of GABABR1 and GluR1 in epileptogenic temporal cortex of patient with post traumatic epilepsy

作　　者：黄永华[1,2] 唐运林 姜富学 方超[1] 竞花兰[1] 

机构地区：[1]中山大学中山医学院法医学系,广东广州510080 [2]广州市刑事刑事科学技术研究所,广东广州510030 [3]广州空军医院,广东广州510602

出　　处：《中国法医学杂志》2011年第4期265-268,共4页Chinese Journal of Forensic Medicine

摘　　要：目的探讨外伤性癫痫患者颞叶癫痫灶组织γ-氨基丁酸B受体亚单位(gamma-aminobutyric acid B receptorsubunit,GABABR1)和谷氨酸受体亚单位(glutamic acid receptor subunit,GluR1)的表达变化,以期为阐释外伤性癫痫发生机制和法医学鉴定提供依据。方法收集15例外伤性癫痫患者脑皮质(外伤性癫痫组)1、5例非外性癫痫患者脑皮质(非外伤性癫痫组)、15例交通事故死亡者脑皮质(交通事故死亡对照组),运用荧光免疫组化技术和RT-PCR技术观察GABABR1和GluR1的表达水平。结果外伤性癫痫组GABAB R1表达水平明显低于对照组,但高于非外伤性癫痫组;外伤性癫痫组GluR1表达水平明显高于对照组,但低于非外伤性癫痫组。结论 GABABR1和GluR1的表达水平的变化在外伤性癫痫病的发病过程中起着一定的作用,可以作为区分外伤性癫痫与非外伤性癫痫的病变化指标之一。Objective To investigate the expression of gamma-aminobutyric acid B receptor subunit（GABABR1） and glutamic acid receptor subunit（GluR1） in epileptogenic temporal cortex of patient with post traumatic epilepsy in order to explain pathogenesis of traumatic epilepsy and provide evidence of medicolegal expertise.Methods All samples of 45 cases were divided into 3 groups（15 cases in each group）： Post traumatic epilepsy（PTE） group come from the patient with post traumatic epilepsy,non-posttraumatic epilepsy（non-PTE） group collected in the patient with non-posttraumatic epilepsy and the control group selected from the autopsied cases who died of traffic accident.GABABR1 and GluR1 expression in temporal cortex of each case was detected with immunofluorescence and RT-PCR.Results The expression of GABABR1 in the PTE group was lower than the control group but higher than the non-PTE group;the expression of GluR1 in the PTE group are higher than the control group but lower than non-PTE group.Conclusion The changes of GABABR1 and GluR1 expression play a certain role in traumatic epilepsy＇s occurrence,which can be used as one of pathologolical targets to distinguish the PTE and non-PTE.

关 键 词：法医病理学 外伤性癫痫 免疫荧光 RT-PCR Γ-氨基丁酸B受体 谷氨酸受体 

分 类 号：D919[医药卫生—法医学]