清化瘀毒方对酒精性大鼠肝纤维化转化生长因子-β1表达的影响  

Effects of Thanh Hoa on the Expression of Alcohol Induced Rat Hepatic Fibrosis Transforming Growth Factor-β_1

作　　者：王邦才[1] 张宇[2] 何国浓[1] 

机构地区：[1]浙江中医药大学附属宁波中医院,浙江宁波315010 [2]浙江中医药大学2007级硕士研究生,浙江杭州310053

出　　处：《中华中医药学刊》2011年第9期2009-2011,共3页Chinese Archives of Traditional Chinese Medicine

基　　金：浙江省中医药科技计划资助项目(2009CA096)

摘　　要：目的:通过观察清化瘀毒方对酒精性大鼠肝纤维化转化生长因子-β1表达的影响,探讨清化瘀毒方抗酒精性肝纤维化的作用机制。方法:选择成年雄性Wistar大鼠,SPF级65只,随机分成正常对照组10只,造模组55只;按照复合因素制作酒精性大鼠肝纤维化模型,造模成功后,造模组采用随机分成3组,分别为复方鳖甲软肝片组10只,清化瘀毒方组10只和模型空白组10只;于第20周末次给药后禁食,全部处死取出肝脏进行HE染色及MASSON染色,免疫组化法检测肝脏组织TGF-β1表达。结果:实验性大鼠肝组织炎症活动度及肝纤维化程度:经清化瘀毒方治疗后,计分均明显降低,与模型空白组比较,差异有显著性意义(P<0.05)。免疫组化法检测酒精性大鼠肝脏TGF-β1的表达:与模型空白组比较,清化瘀毒方组明显降低,差异有显著性意义(P<0.05)。结论:清化瘀毒方能够显著减轻酒精性肝纤维化大鼠肝组织炎症活动度及纤维化程度,并能够降低酒精性肝纤维化大鼠肝组织中TGF-β1表达。Objective： To establish rat models of alcohol induced hepatic fibrosis using composite factor, observe the effect of Thanh Hoa （ qing hua yu du fang） on the expression of hepatic fibrosis transforming growth factor - -β1. To dis- cuss preliminary on the mechanism of anti - alcoholic liver fibrosis function of Thanh Hoa and its validity and reliability in treating alcoholic liver fibrosis. To provide a theoretical foundation for treating liver fibrosis by traditional Chinese medi- cine. Methods： 65 SPF- level male Wistar rats were involved in the study, and were randomly divided into two groups, as 10 in the blank control group and 55 in the model group. Rat models of alcohol induced hepatic fibrosis were estab- lished using composite factors. After the success of modeling, the model group was randomly divided into 3 groups, as 10 in the compound Biejiaruangan tablet group, 10 in the Thanh Hoa group and 10 in the model control group. The compound Biejiaruangan tablet group was gavage fed with the medicine 10ml/kg, the Thanh Hoa group 20ml/kg and the mod- el control group the same amount of saline. At the end of the 20th week, all the rats were sacrificed, HE staining and Masson staining were used for hepatic pathology, and immunohistochemical staining was used for detecting the expression of hepatic TGF-β1 Results ： Significant higher scores of liver inflammation activity and liver fibrosis degree ： Compared to the model control group, the Thanh Hoa group had significant higher scores of liver inflammation activity and liver fibrosis degree （ P 〈0.05 ） ; Significant higher expression of hepatic TGF -β1 ： Compared to the model control group, the Thanh Hoa group had significant higher expression of hepatic TGF -β1 （ P 〈 0. 05）. Conclusion ： Thanh Hoa has the function of reducing liver inflammation activity, fibrosis degree and TGF -β1 expression of alcohol induced rat hepatic fibrosis, this maybe a mechanism of its anti - inflammatory and anti - alcoholic liver fibrosis features.

关 键 词：清化瘀毒方 酒精性肝纤维化 TGF-Β1 

分 类 号：R285.5[医药卫生—中药学]