铜绿假单胞菌β-内酰胺酶基因的检测与分布  

Detection and distribution of β-lactmases-resistant genes of Pseudomonas aeruginosa

作　　者：朱涛[1] 孙各琴[1] 孙运涛[1] 张秀明[1] 张炽伦[1] 王结珍[1] 苏宇霞[1] 李美子[1] 徐全中[1] 

机构地区：[1]中山大学附属中山市人民医院检验医学中心,广东中山528400

出　　处：《中华医院感染学杂志》2011年第17期3545-3548,共4页Chinese Journal of Nosocomiology

基　　金：中山市科技计划项目(20091A034)

摘　　要：目的了解中山地区铜绿假单胞菌β-内酰胺酶类药物的耐药表型和基因分布及其基因型与表型的相关性。方法采用VITEK-2Compact高级专家系统判定2010年1-9月分离的896株铜绿假单胞菌对β-内酰胺酶类药物的耐药表型,用PCR方法检测25株铜绿假单胞菌的13种β-内酰胺酶基因。结果 896株铜绿假单胞菌对β-内酰胺酶类药物的耐药表型共有9种,主要表型为高水平耐药+碳青霉烯酶(不渗透性)占47.3%;连续分离的25株铜绿假单胞菌中耐药表型共有4种,主要表型为高水平耐药+碳青霉烯酶12株占48.0%;检出阳性耐药基因9种:blaOXA-10、blaDHA基因阳性率均为4.0%,blaSHV20.0%,blaCARB12.0%,blaGIM 8.0%,blaGES8.0%,blaTEM16.0%,oprD2基因缺失率60.0%,未检出blaIMP,blaVIM,blaPER,blaVEB,blaSPM。结论临床分离的铜绿假单胞菌β-内酰胺酶类药物耐药表型和基因型基本相符,推测该地区β-内酰胺酶类药物耐药的主要原因是β-内酰胺酶基因的存在导致。OBJECTIVE To investigate the distribution and correlation of β-lactmases drug resistant phenotypes and genotypes of Pseudomonas aeruginosa（PAE） in the zhongshan district.METHODS The senior expert system of VITEK 2 Compact was used to judge the β-lactamase drug resistant phenotypes in 896 strains of PAE from Jan 2010 to Sep 2010,and 13 kinds of β-lactamase genes were detected by PCR in the 25 strains of separated from July to Sep 2010.RESULTS There were 9 species ofβ-lactamase drug-resistant phenotypes modes in 896 strains PAE：the main was high level resistant＋carbapenems（impermeability）（47.3%）;There were 4 species of β-lactamase drug-resistant phenotypes modes in 25 strains PAE separated continuously：high level resistant＋carbapenems（impermeability） accounted for 48%;and 9 kinds of drug resistant genes were positive：blaOXA-10 and blaDHA was 4%,blaSHV 20.0%,a blaCARB 12.0%,blaGIM 8.0%,blaGES 8.0%,blaTEM 16.0%（4/25）,and the rate of oprD2 gene deletion was 60%.blaIMP,blaVIM,blaPER,blaVEB and blaSPM were negative.CONCLUSION The β-lactamase drug resistant phenotypes and genotypes of PAE separated from clinical are basically consistent,which indicate the main reason causes the aminoglycoside drug resistance is the β-lactamase gene.

关 键 词：铜绿假单胞菌 Β-内酰胺酶 耐药 

分 类 号：R378.991[医药卫生—病原生物学]