百草枯中毒大鼠肺组织缺氧诱导因子-1α的表达变化  

HIF-1α expression in lung tissue of rats with paraquat poisoning

作　　者：徐榕[1] 王瑞兰[1] 吴欣[1] 唐雪[1] 

机构地区：[1]上海交通大学附属第一人民医院危重病科,上海201620

出　　处：《中华急诊医学杂志》2011年第9期945-950,共6页Chinese Journal of Emergency Medicine

基　　金：上海市科委生药处项目资助（08411962800）

摘　　要：目的探讨百草枯中毒大鼠肺组织缺氧诱导因子-1α（hypoxia—inducible factor 1 alpha，HIF-1α）的表达变化。方法将96只健康SD大鼠随机（随机数字法）分为对照组和染毒组，染毒组按50mg／kg将20％百草枯（paraquat，PQ）用生理盐水稀释到1mL一次性灌胃染毒灌胃后0，2，6，12，24，48，72h及120h分别收集动脉血做血气分析，肺组织切片做HE染色，Masson染色及免疫组化标记HIF-1α。数据收集后两组之间比较采用单因素方差分析（One—Way ANOVA），相关分析采用Pearson相关分析。结果PQ中毒后2h大鼠肺组织的HIF—1α及胶原的表达即明显升高，低氧血症则在中毒后72h出现。动脉血氧分压与HIF-1α阳性面积/细胞数无显著相关（r=-5．24，P〉0．05）。Masson染色阳性面积／细胞数与HIF-1α阳性面秽细胞数（r=0．819，P〈0．05）呈显著相关。动脉血乳酸与HIF—1α阳性面积／细胞数（r=0．761，P〈0．05）呈显著相关。结论PQ中毒可上调大鼠肺组织的HIF-1α蛋白，且这一变化可能不是由低氧血症介导的。Objective To study the HIF-la expression in rats with paraquat poisoning （PQP）. Methods Ninety-six healthy SD rats were randomly （random number） divided into 2 groups （n = 48 in each group） ： PQP group and control group. The PQP group was further divided into six subgroups as per the different intervals after Paraquat intoxication. Samples from 6 PQP subgroups and control group were taken at 0 h, and 2 h, 6 h, 12 h, 24 h, 48 h, 72 h and 120 h after paraquat administration. Aarterial blood was collected for blood gas analysis, and the sections of lung tissue were stained with hematoxylin-eosin and massong trichrome to detect the trichrome positive area, and HIF-1 c~ was detected by immunohistochemistry. All data were processed with one-factor analysis of variance and Pearson correlation analysis. Results Two hours after paraquat poisoning, the levels of HIF-1α protein and the collagen were both significantly upregulated in the lung tissue in rats with paraquat poisoning. However, the bypoxemia existed until 72h. There was correlation between levels of Masson＇s trichrome positive area and HIF-1α positive area （r = 0. 819, P 〈0. 05）. There was correlation between the levels of lactic acid in blood gas analysis and HIF-1α positive area （r =0. 761, P 〈0. 05. But there was no correlation between levels of PO2 and HIF-1α positive area （ r = - 5.24, P 〉 0.05 ）. Conclusions The level of HIF-1 α was up-regulated in rats with paraquat poisoning and it was not associated with hypoxemia.

关 键 词：百草枯 中毒 缺氧诱导因子-1Α 低氧血症 急性肺损伤 肺间质纤维化 肺泡上皮细胞 

分 类 号：R595.4[医药卫生—内科学]