蓝狐自咬症RAPD标记的筛选  

Screening the RAPD Marker Linked to Self-biting Disease of Blue Fox

作　　者：任二军[1,2] 刘宗岳[1] 张铁涛[3] 邢秀梅[1] 杨福合[1] 

机构地区：[1]中国农业科学院特产研究所吉林省特种经济动物分子生物学重点实验室吉林省特种经济动物分子生物学省部共建实验室,吉林吉林132109 [2]石家庄市农林科学研究院,石家庄050041 [3]中国农业科学院饲料研究所,北京100081

出　　处：《特产研究》2011年第3期1-5,共5页Special Wild Economic Animal and Plant Research

基　　金：国家科技支撑计划项目(2006BAD06B06)

摘　　要：蓝狐是一种珍贵的毛皮动物,自咬症的发生严重影响了蓝狐的生长发育和毛皮质量,给蓝狐养殖业带来了巨大的经济损失。为了寻找与蓝狐自咬症紧密连锁的遗传标记,采用RAPD技术对健康和自咬蓝狐分子标记进行筛选。从120条随机引物中筛选出在健康蓝狐和自咬蓝狐中扩增出差异条带的6条引物,分别为S77、S79、S88、S472、S474、S485。经个体验证,S472、S4852条随机引物在健康蓝狐和自咬蓝狐出现特异条带的个体数量差异达极显著水平(P<0.01),可作为蓝狐自咬症的分子标记,为蓝狐自咬症的深入研究奠定了基础。Blue fox is a precious fur-bearing animal. The self-biting disease of blue foxes can cause serious problem on their growth and the peh-quality, eventually cause considerable economic losses in blue fox farming industry. In order to identify the genetic marker linked to self-biting disease, RAPD was used to analyze molecular marker of the healthy and self-biting blue foxes. 6 primers amplified specific fragments in healthy foxes and self-biting ones were screened from 120 random primers by RAPD, they were S77,S79,S88 ,S472,S474, S485 respectively. Detected by individuals, the numbers with specific fragments amplified by primer S472 and primer S485 in the healthy foxes and self-biting foxes showed significantly difference （P〈0.01）, theses two primers could be used as molecular marker of self-biting disease. The results provided theoretical basis for further study on the self-biting disease of blue foxes.

关 键 词：蓝狐 自咬症 RAPD 分子标记 

分 类 号：Q753[生物学—分子生物学]