利用HPLC/MS对野山参和林下参皂苷的分析  

Ginsenoside Analysis on the Wild Ginseng and the Ginseng Cultivated in Forest by HPLC/MS

作　　者：孙海[1] 张亚玉[1] 王英平[1] 郑培和[1] 

机构地区：[1]中国农业科学院特产研究所,吉林吉林132109

出　　处：《特产研究》2011年第3期52-55,共4页Special Wild Economic Animal and Plant Research

基　　金：国家科技支撑计划项目(2007BAI38B01);吉林省科技支撑项目(20080905)(20090573)

摘　　要：为了明确野山参和林下参中人参皂苷的组成特征及差异所在,利用高效液相色谱-质谱联用仪对野山参和林下参中皂苷进行分析测定。结果表明:野山参和林下参中均含有常见的9种人参皂苷(Re、Rg1、Ro、Rf、Rg2、Rb1、Rc、Rb2、Rd),野山参中还检测到三七皂苷R1、R2和R3以及丙二酰基人参皂苷Rb1和Rc,林下参仅检测到了丙二酰基人参皂苷Rb1、Rc和Rd,没有检测到三七皂苷R1和R3;林下参中Re含量显著高于野山参,平均含量为5.042g/mg,二者达到极显著差异(P<0.01),野山参和林下参中其它8种人参皂苷含量差别不大。The ginsenoside was analyzed by High performance liquid chromatography mass spectrometry detector （HPLC/MS） for evaluated ginsenoside composition characteristics and content diversity in wild gineng and the ginseng cultivated in forests. Results showed that the ginsenoside Re, Rg1, Ro, Rf, Rg3, Rb1, Rc, Rb2 and Rd were detected in different source ginseng, notoginsenoside R1, R2, R3 and propylcne acyl ginseng saponins Rb1, Rc were detected in wild ginseng in the same time, by contrast, propylene acyl ginseng saponins Rb1, Rc and Rd were detected exclusively in wild ginseng under forest and no notoginsenoside R1 and R3. The ginsenoside contents of Rg1, Ro, Rf, Rg2, Rb1, Rc, Rb2 and Rd were similar except Re in wild ginseng and the ginseng cultivated in forest,s ginsenoside Re content in the ginseng cultivated in forests was higher than that of the wild ginseng and the difference was significant in the P〈0.01 level, the average value was 5.042mg/g.

关 键 词：野山参 林下参 人参皂苷 HPLC/MS 

分 类 号：Q503[生物学—生物化学]