腺病毒介导的生长抑制基因4对ECV304细胞体外生长及分泌血管内皮生长因子的影响  

The effect of adenovirus-mediated inhibitor of growth family member 4 on growth and vascular endothelial growth factor secretion of ECV304 cells

作　　者：张海峰[1,2] 盛伟华[2] 缪竞诚[2] 陈卫东 杨吉成[2] 

机构地区：[1]蚌埠医学院第一附属医院肾内科,安徽蚌埠233004 [2]苏州大学医学院细胞与分子生物学教研室,江苏苏州215123

出　　处：《蚌埠医学院学报》2011年第9期931-935,共5页Journal of Bengbu Medical College

基　　金：安徽省高校省级自然科学研究资助项目(KJ2008B082)

摘　　要：目的:检测腺病毒介导的生长抑制基因4(adenovirus-mediated inhibitor of growth family member 4,Ad-ING4)感染后ECV304细胞体外生长及分泌血管内皮生长因子(vascular endothelial growth factor,VEGF)的影响,探讨ING4抑制肿瘤生长的可能机制。方法:构建ING4腺病毒载体,用MTT法检测Ad-ING4感染后ECV304细胞体外生长影响,ELISA检测VEGF的分泌情况。结果:Ad-ING4感染后第3天和第4天ECV304细胞体外生长均受到抑制(P<0.01),第4天时抑制率达63.6%,VEGF分泌受到抑制。结论:ING4能抑制ECV304细胞生长及VEGF分泌。Ad-ING4能抑制血管内皮细胞生长和血管的生成,从而可抑制体内肿瘤的生长。Objective：To interpret the possible anti-tumor mechanism of adenovirus-mediated inhibitor of growth family member 4（Ad-ING4） by testing the growth and vascular endothelial growth factor（VEGF） expression of ECV304 cells infected by Ad-ING4.Methods：Adenovirus vector of ING4 was constructed,then the growth of ECV304 cells was detected by MTT,and the VEGF expression of ECV304 cells infected by Ad-ING4 was detected by ELISA.Results：The VEGF expression of ECV304 cells infected by Ad-ING4 decreased,and the growth of ECV304 was inhibited,and the inhibition ratio was 63.6% on the fourth day.Conclusions：ING4 could down regulate the expression of VEGF,and inhibit the growth of ECV304,and ING4 maybe through this way affect growth,and tumor angiogenesis of lung cancer.

关 键 词：腺病毒感染 生长抑制基因4 血管内皮生长因子 血管生成 

分 类 号：R511.8[医药卫生—内科学]