间日疟原虫MSP1-19基因的克隆与表达  

Cloning and expression of Plasmodium vivax merozoite surface protein-1-19 gene

作　　者：李光友[1] 陈勇[1] 夏惠[2] 方强[2] 刘丹[2] 买月琴[1] 贺文欣[1] 

机构地区：[1]蚌埠医学院免疫学教研室,安徽蚌埠233030 [2]蚌埠医学院病原生物学教研室,安徽蚌埠233030

出　　处：《蚌埠医学院学报》2011年第9期935-937,共3页Journal of Bengbu Medical College

基　　金：卫生部寄生虫病预防与控制技术重点实验室开放课题资助项目(WK008-04)

摘　　要：目的:构建间日疟原虫MSP1-19(Plasmodium vivax merozoite surface protein-1-19,Pv MSP1-19)基因克隆及表达的载体。方法:将MSP1-19基因克隆入原核表达载体pET28a中,构建重组表达载体。以重组载体转化大肠埃希菌BL21,在IPTG诱导下表达重组Pv MSP1-19蛋白。采用亲合层析纯化重组蛋白,应用SDS-PAG电泳和Western blot对表达产物进行鉴定和分析。结果:成功构建了质粒pET28a/Pv MSP1-19,并在大肠埃希菌中诱导可溶性表达的目的蛋白。表达的蛋白能与间日疟患者血清发生特异性结合反应。结论:成功地原核表达出Pv MSP1-19目的蛋白,为间日疟的疫苗研制提供了实验基础。Objective：To construct a vector which clone and express Plasmodium vivax MSP1-19 Gene（Pv MSP1-19）.Methods：The Pv MSP1-19 was cloned into the expression vector pET28a.The recombinant expression vector was transformed into E.coli,and the Pv MSP1-19 protein was expressed under IPTG induction.The recombinant protein was purified by affinity chromatography and the fusion protein was characterized by SDS-PAGE and Western blot.Results：The Pv MSP1-19 gene in plasmid pET28a was expressed in E.coli as a fusion protein.The fusion protein could be reacted specifically with Plasmodium vivax patient serum.Conclusions：The Pv MSP1-19 gene was expressed successfully in E.coli,which provides the necessary basis for preparing vaccine in human.

关 键 词：间日疟 MSP1-19基因 原核表达 基因表达 

分 类 号：R531.31[医药卫生—内科学]