不同提取方法对海燕皂苷抗氧化活性的影响  

Effects of different extraction methods on antioxidant activity of asterosaponin in starfish Asterina pectinifera

作　　者：李敏晶[1,2] 韩艳玲[1] 谭成玉[1,2] 刘远[1,2] 

机构地区：[1]大连海洋大学海洋环境工程学院,辽宁大连116023 [2]大连海洋大学辽宁省高校近岸海洋环境科学与技术重点实验室,辽宁大连116023

出　　处：《大连海洋大学学报》2011年第4期344-347,共4页Journal of Dalian Ocean University

基　　金：辽宁省教育厅高等学校科研计划项目(2009A177)

摘　　要：采用微波辅助萃取法、超声波辅助萃取法和索氏萃取法分别提取海燕Asterina pectinifera总皂苷,用大孔树脂柱层析法对总皂苷进行分离提纯,并将分离提纯后得到的海燕皂苷样品与分离前的总皂苷样品的抗氧化活性进行了测定。结果表明:用不同提取方法所得的海燕皂苷在分离前后抗氧化活性不同,分离纯化后的皂苷样品抗氧化活性较好;3种提取法中用微波辅助萃取法所得海燕皂苷样品的抗氧化活性最好。Asterosaponin were extracted from starfish Asterina pectinifera by microwave-assisted extraction （MAE）, ultrasonicwave-assisted extraction （UAE） and soxhlet extraction method, then isolated and purified by macroporous resin purification method. The antioxidant activity of each asterosaponin was analyzed. The results showed that the antioxidant activity of the asterosaponin was found to be varied with the extraction methods. The maximal antioxidant activity was observed in the purified asterosaponin extracted by MAE.

关 键 词：海燕 皂苷 微波辅助萃取法 超声波辅助萃取法 索氏萃取法 

分 类 号：O629.13[理学—有机化学]