利用NaN_3诱变“中育5号”选育变异种质系及SSR分析  

New Wheat Germplasms from Variational Descendants of 'Zhongyu5' Induced by NaN_3 and Assessment of Genetic Diversity Using SSR Markers

作　　者：张希太 谢淑芹[1] 张彦波[1] 肖磊[1] 张清华[1] 

机构地区：[1]邯郸市农业科学院生物技术中心,河北邯郸056001

出　　处：《西南农业学报》2011年第4期1239-1242,共4页Southwest China Journal of Agricultural Sciences

基　　金：河北省邯郸市科技发展计划项目"利用基因突变技术选育小麦新品种与新资源"(041068)

摘　　要：为创造小麦新种质系,利用诱变剂叠氮化钠并采用"种子处理同时鼓入空气"、"颖苞内滴加"、"生长点注射"3种方法诱变小麦"中育5号"。其中"种子处理同时鼓入空气"方法处理的"中育5号"种子出苗率高,M1代性状变异类型丰富,变异率高,是适合小麦"中育5号"诱变处理的好方法。通过对诱变后代的系统选择,选出了YZY5-1、YZY5-2、YZY5-3 3个稳定的新种质系;利用SSR分子标记检测了"中育5号"及其变异种质系的基因组DNA,引物xgwm148、xgwm428、xgwm260、xgwm469检测出了丰富的多态性片段,从分子水平上证明了叠氮化钠对"中育5号"的诱变效果。In order to create the new wheat germplasms, ‘ Zhongyu5 ’ was induced by NaNa with three ways, including in＇ seed treated by NaN3 with adding air＇, ＇ Na.N3 put in glume＇ ,＇ NaN3 injected into the growing point in stem tip＇. The results showed that ＇ seed treated by NaN3 with adding air＇ was the best way to induce‘ Zhongyu5 ’ . By this way, ‘ Zhongyu5 ’ had high rate of emergence and the variation rate of Ml plants were the highest and the variation type were plenty. Three unaltered germplasm lines including in YZY5-1 ,YZY5-2 and YZY5-3 were selected out by subsequent continuous selection of the variational descendants. DNA of ‘ Zhongyu5 ’ and YZY5-1, YZY5-2, YZY5-3 were detected by SSR markers to select some differential fragments by primer xgwm148, xgwm428, xgwm260, xgwm469. It fu^her attested that YZY5-1 ,YZYS-2 ,YZY5-3 came from ＇ ZhongyuS＇ induced by NaN3 at molecular level. Key words： NaN3 ; Induced variation; Zhongyu5 ; Variation descendants; SSR markers

关 键 词：叠氮化钠 诱变 中育5号 后代变异 SSR分析 

分 类 号：S512.1[农业科学—作物学]