转基因玉米MON863结构特异定量PCR方法的建立  

Establishment of Constructing Specific Quantitative PCR of Genetically Modified Maize (Zea mays L.) Event MON863

作　　者：宋君[1] 王东[1] 游米沙[1] 尹全[1] 刘勇[2] 雷绍荣[1] 向云[1] 

机构地区：[1]四川省农业科学院分析测试中心,四川成都610066 [2]四川省农业科学院植物保护研究所,四川成都61006

出　　处：《西南农业学报》2011年第4期1251-1255,共5页Southwest China Journal of Agricultural Sciences

基　　金：四川省农业科学院青年基金(2009QNJJ-037)

摘　　要：根据转基因玉米MON863载体构建特异结构,设计引物和Taqman探针,建立了转基因玉米MON863载体构建特异结构定量PCR检测方法,并采用该法定量检测了1%含量的MON863标准品(不确定度为10%)。结果显示,采用本文构建的方法获得标准曲线斜率,在-3.6～-3.1之间,相关系数大于0.99,扩增效率为105.436%,在90%～110%的范围内。待检样品的定量检测结果(1.08%)非常接近真实值(1%,不确定度为10%),表明本文建立的转基因玉米MON863结构特异定量PCR检测重复性好,灵敏度和准确度高,可以在日常检验中推广应用。Quantitative PCR detection method of genetically modified maize, event MON863, was established according to the sequence of the vector of the event MON863 and the sample （CRM） contained one percentage of event MON863 component （uncertainty was 10 % ）was tested. The results showed that the slope of standard curve was in the range of - 3.6 - - 3.1. Correlation coemcient was greater than 0.99. The amplified efficiency of this method fell the scope of 90 % - 110 % （ 105.436 % ）. The detection results of sample was 1.08 %, which was closer to the true content （1% ）, indicating that this method had good sensitivity, precision and repeatability.

关 键 词：MON863 zSSⅡb 载体构建特异片段 定量检测 

分 类 号：S513[农业科学—作物学]