撒尔维亚组织培养与快繁技术研究  

Tissue Culture and Rapid Propagation Techniques in Salvia officinalis L.

作　　者：母贵琴[1] 张磊[2] 王萌[2] 王涛[2] 张利[2] 

机构地区：[1]四川农业大学农学院,四川雅安625014 [2]四川农业大学生命科学与理学院,四川雅安625014

出　　处：《西南农业学报》2011年第4期1490-1494,共5页Southwest China Journal of Agricultural Sciences

基　　金：四川省青年科技基金资助项目(08ZQ026-034);四川省科技厅资助项目(07jy029-023);四川省科技厅支撑项目(2008FZ0148);四川省科技厅产学研创新联盟合作项目

摘　　要：以撒尔维亚带芽茎段、叶片、种子为外植体,进行组织培养与快繁技术研究。利用正交试验设置不同激素配比的培养基,对不同外植体分别进行增殖和生根培养,结果表明:撒尔维亚组织培养最适外植体为带芽茎段;芽诱导和增殖的最适培养基为MS+IBA0.50mg/L+KT0.40mg/L+6-BA0.80mg/L;最适生根培养基为1/2 MS+NAA0.50 mg/L+蔗糖20 g.L-1。The tissue culture and rapid propagation techniques were developed by using bud, leaves and seeds as explants in Salvia officina. The three different explants were cultured for proliferation and rooting on the medium with different hormones according to orthogonal design. The results showed that bud was the optimal explants for tissue culture of S. officinalis, the medium of MS ＋ IBA 0.50 mg ·L^-1 ＋ KT 0. 40 mg ·L^-1 ＋6-BA 0.80 mg ·L^-1 was the best for bud differentiation, and the medium of 1/2MS＋ NAA0.50 mg ·L^-1 ＋ sucrose 20 g · L^-1 was the best for rooting,

关 键 词：撒尔维亚 组织培养 快速繁殖 

分 类 号：S567.23[农业科学—中草药栽培]